Anti-NSF Antibody (CAB0926)

Antibody Name:	Anti-NSF Antibody
Antibody SKU:	CAB0926
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat, Zebrafish
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NSF (NP_006169.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat, Zebrafish
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NSF (NP_006169.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGR SMQA ARCP TDEL SLTN CAVV NEKD FQSG QHVI VRTS PNHR YTFT LKTH PSVV PGSI AFSL PQRK WAGL SIGQ EIEV SLYT FDKA KQCI GTMT IEID FLQK KSID SNPY DTDK MAAE FIQQ FNNQ AFSV GQQL VFSF NEKL FGLL VKDI EAMD PSIL KGEP ATGK RQKI EVGL VVGN SQVA FEKA ENSS LNLI GKAK TKEN RQSI INPD WNFE KMGI GGLD KEFS DIFR RAFA SRVF PPEI VEQM GC
Gene ID:	4905
Uniprot:	P46459
Cellular Location:	Cytoplasm
Calculated MW:	71kDa/82kDa
Observed MW:	82kDa

Synonyms:

NSF, SEC18, SKD2

Background:

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

UniProt Protein Function:	NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.
UniProt Protein Details:	Protein type:Autophagy; EC 3.6.4.6; Adaptor/scaffold; Protease; Vesicle Chromosomal Location of Human Ortholog: 17q21 Cellular Component: cytoplasm; cytosol; dendritic shaft; Golgi membrane; Golgi stack; lysosomal membrane; myelin sheath; plasma membrane; postsynaptic density Molecular Function:ATP binding; ATPase activity; ATPase activity, coupled; metal ion binding; PDZ domain binding; protein binding; protein complex binding; protein kinase binding; Rab GTPase binding; syntaxin-1 binding Biological Process: cellular protein metabolic process; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; exocytosis; Golgi to plasma membrane protein transport; Golgi vesicle docking; intra-Golgi vesicle-mediated transport; intracellular protein transport; plasma membrane fusion; positive regulation of protein catabolic process; positive regulation of receptor recycling; post-translational protein modification; potassium ion transport; protein amino acid N-linked glycosylation via asparagine; regulation of exocytosis; synaptic transmission; vesicle-mediated transport
UniProt Code:	P46459
NCBI GenInfo Identifier:	257051048
NCBI Gene ID:	4905
NCBI Accession:	P46459.3
UniProt Secondary Accession:	P46459,Q8N6D7, Q9UKZ2, A8K2D9, B4DFA2,
UniProt Related Accession:	P46459
Molecular Weight:	71,584 Da
NCBI Full Name:	Vesicle-fusing ATPase
NCBI Synonym Full Names:	N-ethylmaleimide sensitive factor
NCBI Official Symbol:	NSF
NCBI Official Synonym Symbols:	SKD2
NCBI Protein Information:	vesicle-fusing ATPase
UniProt Protein Name:	Vesicle-fusing ATPase
UniProt Synonym Protein Names:	N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF
Protein Family:	NSFL1 cofactor
UniProt Gene Name:	NSF
UniProt Entry Name:	NSF_HUMAN

	Western blot analysis of extracts of various cell lines, using NSF antibody (CAB0926) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human liver damage using NSF antibody (CAB0926) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using NSF antibody (CAB0926) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of BALB-3T3 cells using NSF Rabbit pAb (CAB0926) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using NSF Rabbit pAb (CAB0926) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

NSF Antibody (PACO02938)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)