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Anti-DUSP14 Antibody (CAB10287)

SKU:
CAB10287
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 1 Anti-DUSP14 Antibody CAB10287
  • Cell Biology Antibodies 1 Anti-DUSP14 Antibody CAB10287
€139 - €419
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Description

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Antibody Name:Anti-DUSP14 Antibody
Antibody SKU:CAB10287
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human DUSP14 (NP_008957.1).
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Hela, SW480, BT-474, SKOV3, Mouse kidney, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human DUSP14 (NP_008957.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSR GHST LPRT LMAP RMIS EGDI GGIA QITS SLFL GRGS VASN RHLL QARG ITCI VNAT IEIP NFNW PQFE YVKV PLAD MPHA PIGL YFDT VADK IHSV SRKH GATL VHCA AGVS RSAT LCIA YLMK FHNV CLLE AYNW VKAR RPVI RPNV GFWR QLID YERQ LFGK STVK MVQT PYGI VPDV YEKE SRHL MPYW GI
Gene ID:11072
Uniprot:O95147
Cellular Location:
Calculated MW:22kDa
Observed MW:22kDa
Synonyms:DUSP14, MKP-L, MKP6
Background:
UniProt Protein Function:DUSP14: a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (ERK, JNK, p38). Is a negative regulator of T cell costimulation via CD28.
UniProt Protein Details:

Protein type:EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 17q12

Molecular Function:protein binding; RNA binding

NCBI Summary:Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP14 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]
UniProt Code:O95147
NCBI GenInfo Identifier:20137720
NCBI Gene ID:11072
NCBI Accession:O95147.1
UniProt Related Accession:O95147
Molecular Weight:22kDa
NCBI Full Name:Dual specificity protein phosphatase 14
NCBI Synonym Full Names:dual specificity phosphatase 14
NCBI Official Symbol:DUSP14  
NCBI Official Synonym Symbols:MKP6; MKP-L  
NCBI Protein Information:dual specificity protein phosphatase 14
UniProt Protein Name:Dual specificity protein phosphatase 14
UniProt Synonym Protein Names:MKP-1-like protein tyrosine phosphatase; MKP-L; Mitogen-activated protein kinase phosphatase 6; MAP kinase phosphatase 6; MKP-6
Protein Family:Dual specificity protein phosphatase
UniProt Gene Name:DUSP14  
Anti-DUSP14 Antibody (CAB10287)Western blot analysis of extracts of various cell lines, using DUSP14 antibody (CAB10287) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
Anti-DUSP14 Antibody (CAB10287)Immunofluorescence analysis of H9C2 cells using DUSP14 Rabbit pAb (CAB10287) at dilution of 1:100. Blue: DAPI for nuclear staining.

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