Mouse Methionine Adenosyltransferase I Alpha (MAT1a) ELISA Kit
The Mouse Methionine Adenosyltransferase I Alpha (MAT1A) ELISA Kit is specifically designed for the accurate quantification of MAT1A levels in mouse serum, plasma, and tissue lysate samples. This kit ensures high sensitivity and specificity, allowing for precise and reliable results in a variety of research settings.MAT1A is a key enzyme involved in the synthesis of S-adenosylmethionine (SAMe), a crucial methyl donor involved in various biological processes such as DNA methylation, protein methylation, and gene expression regulation.
Dysregulation of MAT1A has been implicated in a variety of diseases, including liver diseases, metabolic disorders, and certain cancers, making it a valuable biomarker for studying these conditions and exploring potential therapeutic strategies.With its user-friendly protocols and high-quality components, the Mouse MAT1A ELISA Kit is an essential tool for researchers looking to investigate the role of MAT1A in health and disease, providing accurate and reliable data for their studies.
Product Name:
Mouse Methionine Adenosyltransferase I Alpha (MAT1a) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
