Anti-SPINT2 Antibody (CAB6749)

Antibody Name:	Anti-SPINT2 Antibody
Antibody SKU:	CAB6749
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-197 of human SPINT2 (NP_066925.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-197 of human SPINT2 (NP_066925.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ADRE RSIH DFCL VSKV VGRC RASM PRWW YNVT DGSC QLFV YGGC DGNS NNYL TKEE CLKK CATV TENA TGDL ATSR NAAD SSVP SAPR RQDS EDHS SDMF NYEE YCTA NAVT GPCR ASFP RWYF DVER NSCN NFIY GGCR GNKN SYRS EEAC MLRC FRQQ ENPP LPLG SK
Gene ID:	10653
Uniprot:	O43291
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	21kDa/28kDa
Observed MW:	34kDa

Synonyms:

SPINT2, DIAR3, HAI-2, HAI2, Kop, PB

Background:

This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SPINT2: Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3); also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Inhibitor; Membrane protein, integral Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: cytoplasm; extracellular region; integral to membrane Molecular Function:serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity Biological Process: cell motility Disease: Diarrhea 3, Secretory Sodium, Congenital, With Or Without Other Congenital Anomalies
NCBI Summary:	This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	O43291
NCBI GenInfo Identifier:	13124550
NCBI Gene ID:	10653
NCBI Accession:	O43291.2
UniProt Secondary Accession:	O43291,O00271, O14895, Q5TZQ3, Q969E0, A8K667, B4DLU1
UniProt Related Accession:	O43291
Molecular Weight:	252
NCBI Full Name:	Kunitz-type protease inhibitor 2
NCBI Synonym Full Names:	serine peptidase inhibitor, Kunitz type, 2
NCBI Official Symbol:	SPINT2
NCBI Official Synonym Symbols:	PB; Kop; HAI2; DIAR3; HAI-2
NCBI Protein Information:	kunitz-type protease inhibitor 2; placental bikunin; serine protease inhibitor, Kunitz type, 2; hepatocyte growth factor activator inhibitor type 2
UniProt Protein Name:	Kunitz-type protease inhibitor 2
UniProt Synonym Protein Names:	Hepatocyte growth factor activator inhibitor type 2; HAI-2; Placental bikunin
Protein Family:	Kunitz-type protease inhibitor
UniProt Gene Name:	SPINT2
UniProt Entry Name:	SPIT2_HUMAN

	Western blot analysis of extracts of MCF-7 cells, using SPINT2 Antibody (CAB6749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human prostate using SPINT2 antibody (CAB6749) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using SPINT2 antibody (CAB6749). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)