Western blot analysis of various lysates, using ABCD1/ALD Rabbit mAb (CAB23569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The ABCD1 / ALD Monoclonal Antibody (CAB23569) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, created using advanced technology, specifically targets the ABCD1 protein, which is mutated in individuals with ALD.By binding to ABCD1, this monoclonal antibody allows for precise detection and analysis of the protein in various sample types, facilitating research on the molecular mechanisms underlying ALD. Its high specificity and sensitivity make it a valuable tool for studies aimed at understanding the pathogenesis of ALD and developing new therapies for this devastating condition.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ABCD1 / ALD Monoclonal Antibody
SKU:
CAB23569
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC3076
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALD, AMN, ALDP, ABC42, ABCD1 / ALD
Positive Sample:
Hep G2, HeLa, NIH/3T3
Cellular Localization:
Multi-Pass Membrane Protein, Peroxisome Membrane.
Calculated MW:
83kDa
Observed MW:
83kDa
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein is likely involved in the peroxisomal transport or catabolism of very long chain fatty acids. Defects in this gene have been identified as the underlying cause of adrenoleukodystrophy, an X-chromosome recessively inherited demyelinating disorder of the nervous system.
Purification Method
Affinity purification
Gene ID
215
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ABCD1 / ALD Rabbit mAb Rabbit mAb (CAB23569) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Western blot analysis of lysates from NIH/3T3 cells, using ABCD1 / ALD Rabbit mAb (CAB23569) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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