Anti-Acetyl-Histone H2B-K5 Antibody (CAB15621)
- SKU:
- CAB15621
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IF
- IP
- ChIP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
Antibody Name: | Anti-Acetyl-Histone H2B-K5 Antibody |
Antibody SKU: | CAB15621 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IHC IF IP ChIP |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B |
Application: | WB IHC IF IP ChIP |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 ChIP 1:50 - 1:100 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | NIH/3T3, C6 |
Immunogen: | A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Gene ID: | 3017/8349 |
Uniprot: | |
Cellular Location: | |
Calculated MW: | |
Observed MW: | 16kDa |
Synonyms: | |
Background: |
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. C6 cells were treated by TSA (1 uM) at 37°C for 18 hoursSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s. | |
Immunohistochemistry of paraffin-embedded rat spleen using Acetyl-Histone H2B-K5 antibody at dilution of 1:100 (40x lens). | |
Immunohistochemistry of paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K5 antibody at dilution of 1:100 (40x lens). | |
Immunohistochemistry of paraffin-embedded mouse spleen using Acetyl-Histone H2B-K5 antibody at dilution of 1:100 (40x lens). | |
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. | |
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. Blue: DAPI for nuclear staining. |