The ACSL5 Antibody (CAB1270) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human ACSL5 samples and has been validated for use in various applications, including Western blotting.ACSL5 is a key player in lipid metabolism, particularly in the activation of long-chain fatty acids for energy production and lipid synthesis. Dysregulation of ACSL5 has been linked to metabolic disorders, such as obesity and diabetes, making it an important target for research in the fields of nutrition, metabolism, and metabolic diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ACSL5 Antibody
SKU:
CAB1270
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACS2, ACS5, FACL5, ACSL5
Positive Sample:
HepG2, HL-60, Mouse liver, Rat liver
Cellular Localization:
Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Mitochondrion, Mitochondrion Outer Membrane, Single-Pass Type Iii Membrane Protein.
Calculated MW:
76kDa
Observed MW:
70-76kDa
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
51703
RRID
AB_2759543
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ACSL5 Rabbit pAb (CAB1270) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
