Anti-ACTL6A Antibody (CAB5387)

Antibody Name:	Anti-ACTL6A Antibody
Antibody SKU:	CAB5387
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6A (NP_004292.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, K-562, MCF7, BT-474, Mouse spleen, Mouse testis, Mouse ovary, Mouse thymus, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACTL6A (NP_004292.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGG VYGG DEVG ALVF DIGS YTVR AGYA GEDC PKVD FPTA IGMV VERD DGST LMEI DGDK GKQG GPTY YIDT NALR VPRE NMEA ISPL KNGM VEDW DSFQ AILD HTYK MHVK SEAS LHPV LMSE APWN TRAK REKL TELM FEHY NIPA FFLC KTAV LTAF ANGR STGL ILDS GATH TTAI PVHD GYVL QQGI VKSP LAGD FITM QCRE LFQE MNIE LVPP YMIA SKEA VREG SPAN WKRK EKLP QVTR SWHN YMCN CVIQ DFQA SVLQ VSDS TYDE QVAA
Gene ID:	86
Uniprot:	O96019
Cellular Location:	Nucleus
Calculated MW:	43kDa/47kDa
Observed MW:	48kDa

Synonyms:

ACTL6A, ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K

Background:

This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.

UniProt Protein Function:	BAF53A: an actin-related protein involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of the helicase SMARCA4 and for association of the SMARCA4 containing remodelling complex BAF with chromatin/nuclear matrix. A component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of specific genes. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 3q26.33 Cellular Component: NuA4 histone acetyltransferase complex; nuclear chromatin; nucleoplasm; nucleus; plasma membrane; protein complex; SWI/SNF complex Molecular Function:chromatin binding; nucleosomal DNA binding; protein binding Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; signal transduction
NCBI Summary:	This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	O96019
NCBI GenInfo Identifier:	23396463
NCBI Gene ID:	86
NCBI Accession:	O96019.1
UniProt Secondary Accession:	O96019,Q8TAE5, Q9BVS8, Q9H0W6, B3KMN1, D3DNR9,
UniProt Related Accession:	O96019
Molecular Weight:	43,236 Da
NCBI Full Name:	Actin-like protein 6A
NCBI Synonym Full Names:	actin like 6A
NCBI Official Symbol:	ACTL6A
NCBI Official Synonym Symbols:	Arp4; ACTL6; BAF53A; INO80K; ARPN-BETA
NCBI Protein Information:	actin-like protein 6A
UniProt Protein Name:	Actin-like protein 6A
UniProt Synonym Protein Names:	53 kDa BRG1-associated factor A; Actin-related protein Baf53a; ArpNbeta; BRG1-associated factor 53A; BAF53A; INO80 complex subunit K
Protein Family:	Actin-like protein
UniProt Gene Name:	ACTL6A
UniProt Entry Name:	ACL6A_HUMAN

	Western blot analysis of extracts of various cell lines, using ACTL6A antibody (CAB5387) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human placenta using ACTL6A Rabbit pAb (CAB5387) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using ACTL6A Rabbit pAb (CAB5387) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using ACTL6A Rabbit pAb (CAB5387) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-ACTL6A Antibody (CAB7709)	ACTL6A Colorimetric Cell-Based ELISA
ACTL6A Antibody (PACO05584)
ACTL6A Antibody (PACO07581)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)