Anti-AKR7A3 Antibody (CAB8194)

Antibody Name:	AKR7A3 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8194
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human AKR7A3 (NP_036199.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HT-29, Mouse kidney, Mouse liver, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human AKR7A3 (NP_036199.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSRQ LSRA RPAT VLGA MEMG RRMD APTS AAVT RAFL ERGH TEID TAFV YSEG QSET ILGG LGLR LGGS DCRV KIDT KAIP LFGN SLKP DSLR FQLE TSLK RLQC PRVD LFYL HMPD HSTP VEET LRAC HQLH QEGK FVEL GLSN YAAW EVAE ICTL CKSN GWIL PTVY QGMY NAIT RQVE TELF PCLR HFGL RFYA FNPL AGGL LTGK YKYE DKNG KQPV GRFF GNTW AEMY RNRY WKEH HFEG IALV EKAL QAAY GASA PSMT SATL RWMY HHSQ LQGA HGDA VILG MSSL EQLE QNLA AAEE GPLE PAVV DAFN QAWH LVTH ECPN YFR
Gene ID:	22977
Uniprot:	O95154
Cellular Location:	Cytoplasm
Calculated MW:	37kDa
Observed MW:	37kDa

Synonyms:	AKR7A3, AFAR2
Background:

UniProt Protein Function:	AKR7A3: Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.-.-.- Chromosomal Location of Human Ortholog: 1p36.13 Cellular Component: cytosol Molecular Function:aldo-keto reductase activity; electron carrier activity; protein binding Biological Process: aldehyde metabolic process; xenobiotic metabolic process
NCBI Summary:	Aldo-keto reductases, such as AKR7A3, are involved in the detoxification of aldehydes and ketones.[supplied by OMIM, Apr 2004]
UniProt Code:	O95154
NCBI GenInfo Identifier:	13123960
NCBI Gene ID:	22977
NCBI Accession:	O95154.2
UniProt Secondary Accession:	O95154,Q86SR4, Q8IVN6, Q8N5V6, Q8TAX1, Q9NUC3,
UniProt Related Accession:	O95154
Molecular Weight:	37,206 Da
NCBI Full Name:	Aflatoxin B1 aldehyde reductase member 3
NCBI Synonym Full Names:	aldo-keto reductase family 7 member A3
NCBI Official Symbol:	AKR7A3
NCBI Official Synonym Symbols:	AFAR2
NCBI Protein Information:	aflatoxin B1 aldehyde reductase member 3
UniProt Protein Name:	Aflatoxin B1 aldehyde reductase member 3
UniProt Synonym Protein Names:	AFB1 aldehyde reductase 2; AFB1-AR 2
Protein Family:	Aflatoxin B1 aldehyde reductase
UniProt Gene Name:	AKR7A3
UniProt Entry Name:	ARK73_HUMAN

	Western blot analysis of extracts of various cell lines, using AKR7A3 antibody (CAB8194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using AKR7A3 antibody (CAB8194) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Secondary Antibody