The ALAS2 Polyclonal Antibody (CAB6522) is a crucial tool for researchers studying ALAS2, an enzyme involved in heme biosynthesis, particularly in the production of the heme precursor, delta-aminolevulinate. This antibody, developed in rabbits, exhibits high reactivity with human samples and has been rigorously validated for use in Western blot applications. By binding specifically to the ALAS2 protein, this antibody allows for precise detection and analysis in various cell types, making it essential for investigations in biochemistry and related fields.ALAS2, or delta-aminolevulinate synthase 2, plays a crucial role in heme production, a critical process for various biological functions, including oxygen transport and energy metabolism.
Dysregulation of ALAS2 has been linked to disorders such as porphyria, a group of rare genetic diseases characterized by defects in heme synthesis. Research into ALAS2 function is vital for understanding the mechanisms underlying these disorders and for developing potential treatments to correct heme synthesis abnormalities. By utilizing the ALAS2 Polyclonal Antibody (CAB6522), researchers can delve deeper into the complexities of heme biosynthesis and its implications for human health.
Product Name:
ALAS2 Rabbit Polyclonal Antibody
SKU:
CAB6522
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-320 of human ALAS2 (NP_001033057.1).
The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
212
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ALAS2 Rabbit pAb (CAB6522) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
