Anti-ALDH2 Antibody (CAB13463)
- SKU:
- CAB13463
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ALDH2 Antibody |
| Antibody SKU: | CAB13463 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human ALDH2 (NP_000681.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse lung, Rat liver, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human ALDH2 (NP_000681.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLRA AARF GPRL GRRL LSAA ATQA VPAP NQQP EVFC NQIF INNE WHDA VSRK TFPT VNPS TGEV ICQV AEGD KEDV DKAV KAAR AAFQ LGSP WRRM DASH RGRL LNRL ADLI ERDR TYLA ALET LDNG KPYV ISYL VDLD MVLK CLRY YAGW ADKY HGKT IPID GDFF SYTR HEPV GVCG QIIP WNFP LLMQ AWKL GPAL ATGN VVVM KVAE QTPL TALY VANL IKEA GFPP GVVN IVPG FGPT AGAA IASH EDVD KVAF TGST EIGR VIQV AAGS SNLK RVTL ELGG KSPN IIMS DADM DWAV EQAH FALF FNQG QCCC AGSR TFVQ EDIY DEFV ERSV ARAK SRVV GNPF DSKT EQGP QVDE TQFK KILG YINT GKQE |
| Gene ID: | 217 |
| Uniprot: | P05091 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 50kDa/56kDa |
| Observed MW: | 54kDa |
| Synonyms: | ALDH2, ALDH-E2, ALDHI, ALDM |
| Background: | This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011] |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerolipid; Lipid Metabolism - fatty acid; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 1.2.1.3; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Mitochondrial; Carbohydrate Metabolism - propanoate; Oxidoreductase; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - butanoate Chromosomal Location of Human Ortholog: 12q24.2 Cellular Component: mitochondrial matrix Molecular Function:aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; alcohol metabolic process; carbohydrate metabolic process; ethanol oxidation; neurotransmitter biosynthetic process Disease: Alcohol Sensitivity, Acute |
| NCBI Summary: | This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Nov 2016] |
| UniProt Code: | P05091 |
| NCBI GenInfo Identifier: | 118504 |
| NCBI Gene ID: | 217 |
| NCBI Accession: | P05091.2 |
| UniProt Related Accession: | P05091 |
| Molecular Weight: | |
| NCBI Full Name: | Aldehyde dehydrogenase, mitochondrial |
| NCBI Synonym Full Names: | aldehyde dehydrogenase 2 family member |
| NCBI Official Symbol: | ALDH2Â Â |
| NCBI Official Synonym Symbols: | ALDM; ALDHI; ALDH-E2Â Â |
| NCBI Protein Information: | aldehyde dehydrogenase, mitochondrial |
| UniProt Protein Name: | Aldehyde dehydrogenase, mitochondrial |
| UniProt Synonym Protein Names: | ALDH class 2; ALDH-E2; ALDHI |
| Protein Family: | NADP-dependent fatty aldehyde dehydrogenase |
| UniProt Gene Name: | ALDH2Â Â |
| UniProt Entry Name: | ALDH2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ALDH2 antibody (CAB13463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using ALDH2 antibody (CAB13463) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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