Western blot analysis of extracts of various cell lines, using Alpha2-adaptin/AP2A2 antibody (CAB21978) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Alpha2-adaptin/AP2A2 Monoclonal Antibody (CAB21978) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced through monoclonal techniques, is highly specific for the alpha2 adaptin protein and is validated for use in a variety of applications, including immunofluorescence and flow cytometry.The alpha2 adaptin protein is a key component of the AP2 complex, which plays a crucial role in clathrin-mediated endocytosis and vesicle formation. By targeting the alpha2 adaptin protein, this antibody allows for the visualization and analysis of AP2 complex activity in cells, providing valuable insights into cellular trafficking pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Alpha2-adaptin/AP2A2 Monoclonal Antibody
SKU:
CAB21978
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC54447
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.
Purification Method
Affinity purification
Gene ID
161
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Alpha2-adaptin/AP2A2 Rabbit mAb (CAB21978) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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