Anti-ANGPTL3 Antibody (CAB0689)

Antibody Name:	Anti-ANGPTL3 Antibody
Antibody SKU:	CAB0689
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-460 of human ANGPTL3 (NP_055310.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Mouse
Positive Samples:	Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 231-460 of human ANGPTL3 (NP_055310.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LNEI RNVK HDGI PAEC TTIY NRGE HTSG MYAI RPSN SQVF HVYC DVIS GSPW TLIQ HRID GSQN FNET WENY KYGF GRLD GEFW LGLE KIYS IVKQ SNYV LRIE LEDW KDNK HYIE YSFY LGNH ETNY TLHL VAIT GNVP NAIP ENKD LVFS TWDH KAKG HFNC PEGY SGGW WWHD ECGE NNLN GKYN KPRA KSKP ERRR GLSW KSQN GRLY SIKS TKML IHPT DSES FE
Gene ID:	27329
Uniprot:	Q9Y5C1
Cellular Location:	Secreted
Calculated MW:	53kDa
Observed MW:	53kDa

Synonyms:

ANGPTL3, ANG-5, ANGPT5, ANL3, FHBL2

Background:

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2.

UniProt Protein Function:	ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.
UniProt Protein Details:	Protein type:Secreted; Cell adhesion; Inhibitor; Motility/polarity/chemotaxis; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1p31.3 Cellular Component: extracellular space; cell surface Molecular Function:integrin binding; enzyme inhibitor activity; phospholipase inhibitor activity; growth factor activity Biological Process: integrin-mediated signaling pathway; cholesterol metabolic process; phospholipid catabolic process; glycerol metabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; sequestering of lipid; positive regulation of angiogenesis; cholesterol homeostasis; acylglycerol homeostasis; phospholipid metabolic process; artery morphogenesis; negative regulation of lipoprotein lipase activity; positive regulation of cell migration; phospholipid homeostasis Disease: Hypobetalipoproteinemia, Familial, 2
NCBI Summary:	This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Aug 2015]
UniProt Code:	Q9Y5C1
NCBI GenInfo Identifier:	25008126
NCBI Gene ID:	27329
NCBI Accession:	Q9Y5C1.1
UniProt Related Accession:	Q9Y5C1
Molecular Weight:	~ 54kDa
NCBI Full Name:	Angiopoietin-related protein 3
NCBI Synonym Full Names:	angiopoietin like 3
NCBI Official Symbol:	ANGPTL3
NCBI Official Synonym Symbols:	ANL3; ANG-5; FHBL2; ANGPT5
NCBI Protein Information:	angiopoietin-related protein 3
UniProt Protein Name:	Angiopoietin-related protein 3
UniProt Synonym Protein Names:	Angiopoietin-5; ANG-5; Angiopoietin-like protein 3
Protein Family:	Angiopoietin-related protein
UniProt Gene Name:	ANGPTL3
UniProt Entry Name:	ANGL3_HUMAN

	Western blot analysis of extracts of various cell lines, using ANGPTL3 antibody (CAB0689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse kidney using ANGPTL3 Antibody (CAB0689) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-ANGPTL3 Antibody (CAB5225)	Human Angiopoietin-like 3 / ANGPTL3 ELISA Kit
ANGPTL3 Antibody (PACO07733)	Human ANGPTL3 (Angiopoietin Like Protein 3) CLIA Kit (HUES00238)
	Human ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit (HUES01555)
	Human Angiopoietin-related protein 3 (ANGPTL3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)