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Anti-HNF-4-alpha Antibody (CAB20865)

SKU:
CAB20865
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
€179 - €469
Frequently bought together:

Description

Antibody Name:Anti-HNF-4-alpha Antibody (CAB20865)
Antibody SKU:CAB20865
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Mouse HNF-4-alpha.
Application:Western blotting, Immunohistochemistry
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
ChIP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Rat liver
Immunogen:Recombinant protein of Mouse HNF-4-alpha.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Nucleus
Calculated MW:51kDa/52kDa
Observed MW:53KDa
Synonyms:HNF4A, FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, HNF4a
Background:The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.
Western blot analysis of extracts of various cell lines, using HNF-4-alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Western blot analysis of extracts of various cell lines, using HNF-4-alpha antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

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