The Anti-Human CD3 FITC, CD4 PE, CD45 PerCP-Cyanine5.5 Cocktail is a powerful tool for immunophenotyping studies, offering a comprehensive view of T cell populations in human samples. This cocktail of fluorescently-conjugated antibodies targets key cell surface markers involved in T cell activation and function, providing researchers with valuable insights into the immune response.The combination of CD3, CD4, and CD45 antibodies allows for the identification and analysis of T cell subsets, including helper T cells, cytotoxic T cells, and memory T cells. The FITC, PE, and PerCP-Cyanine5.
5 conjugates provide bright and specific staining, enabling robust detection and quantification of T cell populations in flow cytometry experiments.By utilizing this antibody cocktail, researchers can gain a deeper understanding of T cell dynamics in various disease states, including cancer, autoimmune disorders, and infectious diseases. The ability to simultaneously analyze multiple T cell markers in a single experiment makes this antibody cocktail a valuable tool for immunology research and drug development efforts targeting T cell-mediated immunity.
For whole blood samples, add 5 μL Anti-Human CD3-FITC/CD4-PE/CD45-PerCP-Cyanine5.5 Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×106 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD3-FITC/CD4-PE/CD45-PerCP- Cyanine5.5 Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
Spectrum:
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
This product is a FCM antibody cocktail made up of FITC Anti-Human CD3 Antibody [Clone: OKT-3] (Mouse IgG2a, κ),PE Anti-Human CD4 Antibody [Clone: RPA-T4] (Mouse IgG1, κ) and PerCP/Cyanine5.5 Anti-Human CD45 Antibody [Clone: HI30] (Mouse IgG1, κ).; CD3 is a heterotetrameric protein consisting of a CD3γ, a CDδ and 2 CD3ε. It forms complex with TCR. OKT-3 recognize human CD3ε. Human CD3 is expressed on the surface of T cells and NKT cells.; CD4 is also called Leu-3 or T4. It's a single-chain type I transmembrane glycoprotein, mainly expressed on the surface of T cells, and monocytes/macrophages. In T cells, CD4 forms complex with TCR/CD3 and play important roles in T cell immunity. The target of HIV is CD4+ T cells. Reduction of CD4+ T cells is the main reason of defected immunity after HIV infection.; CD45 is a single-chain type I transmembrane glycoprotein. Except for erythrocytes and platelets, CD45 is expressed on nearly all of the hematopoietic cells with high level. It is a common marker for blood leukocytes. CD45 is a receptor type protein tyrosine phophatase and plays essential roles in B cell and T cells signaling.
