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Western blot analysis of various lysates, using MTAP Rabbit pAb (CAB21496) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

MTAP Polyclonal Antibody (CAB21496)

Datasheet MSDS
SKU CAB21496
Product Type Antibody
Antibody Type Polyclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms BDMF, MSAP, DMSFH, LGMBF, DMSMFH, c86fus, HEL-249, MTAP
$127 - $488

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Description

system_update_altDatasheetsystem_update_altMSDS

MTAP Polyclonal Antibody (CAB21496)

The MTAP Polyclonal Antibody (CAB21496) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, offers high specificity and sensitivity for detecting MTAP in human samples, making it ideal for use in Western blotting, immunohistochemistry, and other research applications.MTAP plays a critical role in cell metabolism, influencing processes such as DNA synthesis and repair. Dysregulation of MTAP has been linked to various cancers, making it a promising target for cancer research and drug development.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:MTAP Polyclonal Antibody
SKU:CAB21496
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MASG TTTT AVKI GIIG GTGL DDPE ILEG RTEK YVDT PFGK PSDA LILG KIKN VDCV LLAR HGRQ HTIM PSKV NYQA NIWA LKEE GCTH VIVT TACG SLRE EIQP GDIV IIDQ FIDR TTMR PQSF YDGS HSCA RGVC HIPM AEPF CPKT REVL IETA KKLG LRCH SKGT MVTI EGPR FSSR AESF MFRT WGAD VINM TTVP EVVL AKEA GICY ASIA MATD YDCW KEHE EAVS VDRV LKTL KENA NKAK SLLL TTIP QIGS TEWS ETLH NLKN MAQF SVLL PRH
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:100 - 1:500
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BDMF, MSAP, DMSFH, LGMBF, DMSMFH, c86fus, HEL-249, MTAP
Positive Sample:HT-29, NIH/3T3
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:31kDa
Observed MW:31kDa

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage pathway of both adenine and methionine. The encoded enzyme is deficient in many cancers. Multiple alternatively spliced transcript variants have been described for this gene.

Purification MethodAffinity purification
Gene ID4507
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using MTAP Rabbit pAb (CAB21496) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunofluorescence analysis of HeLa cells using MTAP Rabbit pAb (CAB21496) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of PC-12 cells using MTAP Rabbit pAb (CAB21496) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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