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Anti-Pumilio 1 Antibody (CAB0571)

SKU:
CAB0571
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
HSPUM
PUMH
PUMH1
PUML1
Research Area:
Developmental Biology
€139 - €419
Frequently bought together:

Description

Product Name:Pumilio 1 Rabbit mAb
Product Code:CAB0571
Size:20uL, 50uL, 100uL
Synonyms:HSPUM, PUMH, PUMH1, PUML1
Applications:WB, IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Pumilio 1
Applications:WB, IF
Recommended Dilutions:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Jurkat, Mouse testis
Immunogen:A synthesized peptide derived from human Pumilio 1
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9698
Uniprot:Q14671
Cellular Location:Cytoplasm, Cytoplasmic granule, P-body
Calculated MW:140kDa
Observed MW:140kDa
UniProt Protein Function:Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation.
NCBI Summary:This gene encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:Q14671
NCBI GenInfo Identifier:41688619
NCBI Gene ID:9698
NCBI Accession:Q14671.3
UniProt Secondary Accession:Q14671,Q53HH5, Q5VXY7, Q9HAN1, A8K6W4, B4DG92, D3DPN3 E9PCJ0,
UniProt Related Accession:Q14671
Molecular Weight:119,558 Da
NCBI Full Name:Pumilio homolog 1
NCBI Synonym Full Names:pumilio RNA binding family member 1
NCBI Official Symbol:PUM1  
NCBI Official Synonym Symbols:PUMH; HSPUM; PUMH1; PUML1  
NCBI Protein Information:pumilio homolog 1
UniProt Protein Name:Pumilio homolog 1
Protein Family:Pumilio
UniProt Gene Name:PUM1  
UniProt Entry Name:PUM1_HUMAN

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