Anti-APOL1 Antibody (CAB7943)

Antibody Name:	Anti-APOL1 Antibody
Antibody SKU:	CAB7943
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 179-398 of human APOL1 (NP_003652.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	Raji, Jurkat, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 179-398 of human APOL1 (NP_003652.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SGSL SISS GILT LVGM GLAP FTEG GSLV LLEP GMEL GITA ALTG ITSS TMDY GKKW WTQA QAHD LVIK SLDK LKEV REFL GENI SNFL SLAG NTYQ LTRG IGKD IRAL RRAR ANLQ SVPH ASAS RPRV TEPI SAES GEQV ERVN EPSI LEMS RGVK LTDV APVS FFLV LDVV YLVY ESKH LHEG AKSE TAEE LKKV AQEL EEKL NILN NNYK ILQA DQEL
Gene ID:	8542
Uniprot:	O14791
Cellular Location:	Secreted
Calculated MW:	42kDa/43kDa/45kDa
Observed MW:	38kDa

Synonyms:

APOL1, APO-L, APOL, APOL-I, FSGS4

Background:

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Channel, chloride; Secreted; Secreted, signal peptide; Lipid-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: extracellular region; extracellular space; intrinsic to membrane Molecular Function:chloride channel activity; lipid binding; protein binding Biological Process: cytolysis; innate immune response; killing of cells of another organism; receptor-mediated endocytosis Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To
NCBI Summary:	This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
UniProt Code:	O14791
NCBI GenInfo Identifier:	206729940
NCBI Gene ID:	8542
NCBI Accession:	O14791.5
UniProt Secondary Accession:	O14791,O60804, Q5R3P7, Q5R3P8, Q96AB8, Q96PM4, Q9BQ03 A5PLQ4, B4DU12, E9PF24,
UniProt Related Accession:	O14791
Molecular Weight:	42,158 Da
NCBI Full Name:	Apolipoprotein L1
NCBI Synonym Full Names:	apolipoprotein L1
NCBI Official Symbol:	APOL1
NCBI Official Synonym Symbols:	APOL; APO-L; FSGS4; APOL-I
NCBI Protein Information:	apolipoprotein L1
UniProt Protein Name:	Apolipoprotein L1
UniProt Synonym Protein Names:	Apolipoprotein L; Apo-L; ApoL; Apolipoprotein L-I; ApoL-I
Protein Family:	Apolipoprotein
UniProt Gene Name:	APOL1
UniProt Entry Name:	APOL1_HUMAN

	Western blot analysis of extracts of various cell lines, using APOL1 antibody (CAB7943) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using APOL1 antibody (CAB7943) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-APOL1 Antibody (CAB13376)	Human Apolipoprotein L1 / APOL1 ELISA Kit
Anti-APOL1 Antibody (CAB4412)	Human Apolipoprotein L1 (APOL1) ELISA Kit
APOL1 Antibody (PACO00408)
APOL1 Antibody (PACO07796)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)