Anti-ATP6V0D1 Antibody (CAB4271)

Antibody Name:	Anti-ATP6V0D1 Antibody
Antibody SKU:	CAB4271
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATP6V0D1 (NP_004682.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ATP6V0D1 (NP_004682.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSFF PELY FNVD NGYL EGLV RGLK AGVL SQAD YLNL VQCE TLED LKLH LQST DYGN FLAN EASP LTVS VIDD RLKE KMVV EFRH MRNH AYEP LASF LDFI TYSY MIDN VILL ITGT LHQR SIAE LVPK CHPL GSFE QMEA VNIA QTPA ELYN AILV DTPL AAFF QDCI SEQD LDEM NIEI IRNT LYKA YLES FYKF CTLL GGTT ADAM CPIL EFEA DRRA FIIT INSF GTEL SKED RAKL FPHC GRLY PEGL AQLA RADD YEQV KNVA DYYP EYKL LFEG AGSN PGDK TLED RFFE HEVK LNKL AFLN QFHF GVFY AFVK LKEQ ECRN IVWI AECI AQRH RAKI DNYI PIF
Gene ID:	9114
Uniprot:	P61421
Cellular Location:	Cytoplasmic side, Membrane, Peripheral membrane protein
Calculated MW:	40kDa
Observed MW:	40kDa

Synonyms:

ATP6V0D1, ATP6D, ATP6DV, P39, VATX, VMA6, VPATPD

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.

UniProt Protein Function:	ATP6V0D1: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. Belongs to the V-ATPase V0D/AC39 subunit family.
UniProt Protein Details:	Protein type:Hydrolase; Vesicle; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: centrosome; phagocytic vesicle membrane; synaptic vesicle; membrane; lysosomal membrane; apical plasma membrane; early endosome; endosome membrane; vacuolar proton-transporting V-type ATPase complex; nerve terminal Molecular Function:hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding; protein complex binding Biological Process: interaction with host; proton transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; cilium biogenesis; brain development; transmembrane transport
NCBI Summary:	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [provided by RefSeq, Jul 2008]
UniProt Code:	P61421
NCBI GenInfo Identifier:	47606646
NCBI Gene ID:	9114
NCBI Accession:	P61421.1
UniProt Secondary Accession:	P61421,P12953, Q02547,
UniProt Related Accession:	P61421
Molecular Weight:	351
NCBI Full Name:	V-type proton ATPase subunit d 1
NCBI Synonym Full Names:	ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
NCBI Official Symbol:	ATP6V0D1
NCBI Official Synonym Symbols:	P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD
NCBI Protein Information:	V-type proton ATPase subunit d 1; V-ATPase, subunit D; V-ATPase subunit d 1; V-ATPase AC39 subunit; 32 kDa accessory protein; vacuolar proton pump subunit d 1; V-ATPase 40 KDa accessory protein; H(+)-transporting two-sector ATPase, subunit D; ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D
UniProt Protein Name:	V-type proton ATPase subunit d 1
UniProt Synonym Protein Names:	32 kDa accessory protein; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; p39; Vacuolar proton pump subunit d 1
Protein Family:	V-type proton ATPase
UniProt Gene Name:	ATP6V0D1
UniProt Entry Name:	VA0D1_HUMAN

Western blot analysis of extracts of HeLa cells, using ATP6V0D1 antibody (CAB4271) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

ATP6V0D1 Antibody (PACO01745)

ATP6V0D1 Antibody (PACO07932)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)