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BORA Rabbit Polyclonal Antibody (CAB13847)

SKU:
CAB13847
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
€139 - €419
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Description

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Product Name:BORA Rabbit Polyclonal Antibody
SKU:CAB13847
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 320-619 of human BORA (NP_079084.3).
Sequence:KYSL GSIT SPSP ISSP TFSP IEFQ IGET PLSE QRKF TVHS PDAS SGTN SNGI TNPC IRSP YIDG CSPI KNWS PMRL QMYS GGTQ YRTS VIQI PFTL ETQG EDEE DKEN IPST DVSS PAMD AAGI HLRQ FSNE ASTH GTHL VVTA MSVT QNQS SASE KELA LLQD VERE KDNN TVDM VDPI EIAD ETTW IKEP VDNG SLPM TDFV SGIA FSIE NSHM CMSP LAES SVIP CESS NIQM DSGY NTQN CGSN IMDT VGAE SYCK ESDA QTCE VESK SQAF NMKQ DHTT QRCW MKTA SPFQ CSSP
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:C13orf34; BORA
Positive Sample:HT-29,HeLa
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, meiotic spindle, nucleus
Calculated MW:61kDa
Observed MW:90kDa

BORA is an activator of the protein kinase Aurora A (AURKA; MIM 603072), which is required for centrosome maturation, spindle assembly, and asymmetric protein localization during mitosis (Hutterer et al., 2006 [PubMed 16890155]).

Purification Method:Affinity purification
Gene ID:79866
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using BORA Rabbit pAb (CAB13847) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.

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