Anti-BPGM Antibody (CAB7880)

Antibody Name:	Anti-BPGM Antibody
Antibody SKU:	CAB7880
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human BPGM (NP_001715.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Jurkat, HeLa, Mouse liver, Mouse spleen, Mouse testis, Mouse skeletal muscle, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human BPGM (NP_001715.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKY KLIM LRHG EGAW NKEN RFCS WVDQ KLNS EGME EARN CGKQ LKAL NFEF DLVF TSVL NRSI HTAW LILE ELGQ EWVP VESS WRLN ERHY GALI GLNR EQMA LNHG EEQV RLWR RSYN VTPP PIEE SHPY YQEI YNDR RYKV CDVP LDQL PRSE SLKD VLER LLPY WNER IAPE VLRG KTIL ISAH GNSS RALL KHLE GISD EDII NITL PTGV PILL ELDE NLRA VGPH QFLG DQEA IQAA IKKV EDQG KVKQ AKK
Gene ID:	669
Uniprot:	P07738
Cellular Location:
Calculated MW:	30kDa
Observed MW:	30kDa

Synonyms:

BPGM, DPGM

Background:

2, 3-diphosphoglycerate (2, 3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2, 3-DPG synthesis via its synthetase activity, and 2, 3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	BPGM: Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of its allosteric effector 2,3- bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD). A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.
UniProt Protein Details:	Protein type:EC 5.4.2.4; EC 3.1.3.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); EC 5.4.2.11; Isomerase Chromosomal Location of Human Ortholog: 7q33 Molecular Function:phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity Biological Process: erythrocyte development; glycolysis; dephosphorylation; carbohydrate metabolic process; respiratory gaseous exchange Disease: Bisphosphoglycerate Mutase Deficiency
NCBI Summary:	2,3-diphosphoglycerate (2,3-DPG) is a small molecule found at high concentrations in red blood cells where it binds to and decreases the oxygen affinity of hemoglobin. This gene encodes a multifunctional enzyme that catalyzes 2,3-DPG synthesis via its synthetase activity, and 2,3-DPG degradation via its phosphatase activity. The enzyme also has phosphoglycerate phosphomutase activity. Deficiency of this enzyme increases the affinity of cells for oxygen. Mutations in this gene result in hemolytic anemia. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009]
UniProt Code:	P07738
NCBI GenInfo Identifier:	130350
NCBI Gene ID:	669
NCBI Accession:	P07738.2
UniProt Related Accession:	P07738
Molecular Weight:	30kDa
NCBI Full Name:	Bisphosphoglycerate mutase
NCBI Synonym Full Names:	bisphosphoglycerate mutase
NCBI Official Symbol:	BPGM
NCBI Official Synonym Symbols:	DPGM; ECYT8
NCBI Protein Information:	bisphosphoglycerate mutase
UniProt Protein Name:	Bisphosphoglycerate mutase
UniProt Synonym Protein Names:	2,3-bisphosphoglycerate mutase, erythrocyte; 2,3-bisphosphoglycerate synthase (EC:3.1.3.13, EC:5.4.2.11); 2,3-diphosphoglycerate mutase; DPGM; BPG-dependent PGAM
Protein Family:	Bisphosphoglycerate mutase
UniProt Gene Name:	BPGM
UniProt Entry Name:	PMGE_HUMAN

	Western blot analysis of extracts of various cell lines, using BPGM antibody (CAB7880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using BPGM antibody (CAB7880) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using BPGM Rabbit pAb (CAB7880) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

BPGM Antibody (PACO08036)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)