Western blot analysis of Mouse liver, using Complement C3 antibody (CAB22265) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Complement C3 Monoclonal Antibody (CAB22265) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using recombinant technology, specifically targets the Complement C3 protein, which plays a crucial role in the complement cascade and immune response.The antibody is highly specific to human samples and is suitable for use in various applications, including Western blotting and immunohistochemistry. Its high binding affinity allows for precise detection and analysis of Complement C3 in different cell types and tissues.Complement C3 is essential for both innate and adaptive immune responses, with dysregulation of its activity implicated in various diseases, including autoimmune disorders and inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Complement C3 Monoclonal Antibody
SKU:
CAB22265
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC56851
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes complement protein C3 which plays a central role in the classical, alternative and lectin activation pathways of the complement system. The encoded preproprotein undergoes a multi-step processing to generate various functional peptides. Mice deficient in the encoded protein fail to clear bacteria from the blood stream upon infection, display diminished airway hyperresponsiveness and lung eosinophilia upon allergen-induced pulmonary allergy, and develop severe lung injury after deposition of IgG immune complexes. Deficiency of the homolog of the encoded protein in humans was found to be associated with increased susceptibility to infections, age-related macular degeneration, and atypical hemolytic uremic syndrome.
Purification Method
Affinity purification
Gene ID
12266
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse liver, using Complement C3 Rabbit mAb (CAB22265) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates, using Complement C3 Rabbit mAb (CAB22265) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
