Anti-CCT2 Antibody (CAB6546)

Antibody Name:	Anti-CCT2 Antibody
Antibody SKU:	CAB6546
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human CCT2 (NP_006422.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, SKOV3, A-549, HT-29, Mouse spleen, Mouse ovary, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human CCT2 (NP_006422.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GGSL ADSY LDEG FLLD KKIG VNQP KRIE NAKI LIAN TGMD TDKI KIFG SRVR VDST AKVA EIEH AEKE KMKE KVER ILKH GINC FINR QLIY NYPE QLFG AAGV MAIE HADF AGVE RLAL VTGG EIAS TFDH PELV KLGS CKLI EEVM IGED KLIH FSGV ALGE ACTI VLRG ATQQ ILDE AERS LHDA LCVL AQTV KDSR TVYG GGCS EMLM AHAV TQLA NRTP GKEA VAME SYAK ALRM LPTI IADN AGYD SADL VAQL RAAH SEGN TTAG LDMR EGTI GDMA ILGI TESF QVKR QVLL SAAE AAEV ILRV DNII KAAP RKRV PDHH PC
Gene ID:	10576
Uniprot:	P78371
Cellular Location:	Cytoplasm
Calculated MW:	52kDa/57kDa
Observed MW:	57kDa

Synonyms:

CCT2, 99D8.1, CCT-beta, CCTB, HEL-S-100n, PRO1633, TCP-1-beta

Background:

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CCT-beta: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Belongs to the TCP-1 chaperonin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Chaperone Chromosomal Location of Human Ortholog: 12q15 Cellular Component: chaperonin-containing T-complex; microtubule; cytoplasm; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; chaperone-mediated protein complex assembly
NCBI Summary:	The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:	P78371
NCBI GenInfo Identifier:	6094436
NCBI Gene ID:	10576
NCBI Accession:	P78371.4
UniProt Secondary Accession:	P78371,Q14D36, Q6IAT3, A8K402, B5BTY7, B7Z243, B7Z7K4 B7ZAT2,
UniProt Related Accession:	P78371
Molecular Weight:	535
NCBI Full Name:	T-complex protein 1 subunit beta
NCBI Synonym Full Names:	chaperonin containing TCP1, subunit 2 (beta)
NCBI Official Symbol:	CCT2
NCBI Official Synonym Symbols:	CCTB; 99D8.1; PRO1633; CCT-beta; HEL-S-100n; TCP-1-beta
NCBI Protein Information:	T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; epididymis secretory sperm binding protein Li 100n; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing t-complex polypeptide 1, beta subunit
UniProt Protein Name:	T-complex protein 1 subunit beta
UniProt Synonym Protein Names:	CCT-beta
Protein Family:	Choline-phosphate cytidylyltransferase
UniProt Gene Name:	CCT2
UniProt Entry Name:	TCPB_HUMAN

	Western blot analysis of extracts of various cell lines, using CCT2 antibody (CAB6546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of HeLa cells using CCT2 antibody (CAB6546). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug CCT2 antibody (CAB6546). Western blot was performed from the immunoprecipitate using CCT2 antibody (CAB6546) at a dilition of 1:1000.

Antibodies

Anti-TCP1 beta Antibody (CAB4700)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)