Western blot analysis of U-937, using CD97 antibody (CAB22218) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:200000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The CD97 Monoclonal Antibody (CAB22218) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal techniques, shows high specificity and sensitivity towards human CD97, making it a reliable option for Western blot and immunohistochemistry applications.CD97 is known for its involvement in cell adhesion, migration, and signaling, making it a key player in processes such as embryonic development, inflammation, and cancer progression. By targeting CD97 with this monoclonal antibody, researchers can detect and quantify CD97 expression in different cell types, providing valuable insights into its role in different biological contexts.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD97 Monoclonal Antibody
SKU:
CAB22218
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC55829
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19.
Purification Method
Affinity purification
Gene ID
976
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-3 cells using CD97 Rabbit mAb (CAB22218) at 1:2000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
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