Anti-CLDN3 Antibody (CAB2946)

Antibody Name:	Anti-CLDN3 Antibody
Antibody SKU:	CAB2946
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human CLDN3

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HT-29, Mouse intestine

Immunogen:	Recombinant protein of human CLDN3
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	1365
Uniprot:	O15551
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, tight junction
Calculated MW:	23kDa
Observed MW:	23kDa

Synonyms:

CLDN3, C7orf1, CPE-R2, CPETR2, HRVP1, RVP1, claudin-3

Background:

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat.

UniProt Protein Function:	Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the claudin family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Cytoskeletal Chromosomal Location of Human Ortholog: 7q11.23 Cellular Component: apicolateral plasma membrane; integral to membrane; integral to plasma membrane; lateral plasma membrane; tight junction Molecular Function:identical protein binding; structural molecule activity; transmembrane receptor activity Biological Process: calcium-independent cell-cell adhesion; protein heterooligomerization; protein homooligomerization; response to hypoxia; signal transduction
NCBI Summary:	Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]
UniProt Code:	O15551
NCBI GenInfo Identifier:	6685273
NCBI Gene ID:	1365
NCBI Accession:	O15551.1
UniProt Secondary Accession:	O15551,Q9Z0G9, Q63400,
UniProt Related Accession:	O15551
Molecular Weight:	23,319 Da
NCBI Full Name:	Claudin-3
NCBI Synonym Full Names:	claudin 3
NCBI Official Symbol:	CLDN3
NCBI Official Synonym Symbols:	RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
NCBI Protein Information:	claudin-3
UniProt Protein Name:	Claudin-3
UniProt Synonym Protein Names:	Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1
Protein Family:	Claudin
UniProt Gene Name:	CLDN3
UniProt Entry Name:	CLD3_HUMAN

	Western blot analysis of extracts of various cell lines, using CLDN3 antibody (CAB2946) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse stomach using CLDN3 antibody (CAB2946) (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using CLDN3 antibody (CAB2946) (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using CLDN3 antibody (CAB2946) (40x lens).

Antibodies	ELISA Kits
Anti-CLDN3 Antibody (CAB11650)	Human CLDN3 / Claudin-3 ELISA Kit
CLDN3 Antibody (PACO00607)	Human CLDN3 (Claudin 3) CLIA Kit (HUES00473)
Phospho-CLDN3 (Y219) Antibody (PACO03480)	Human CLDN3 (Claudin 3) ELISA Kit (HUES01898)
CLDN3 Antibody (PACO07131)	Human Claudin-3 (CLDN3) ELISA Kit
CLDN3 Antibody (PACO08458)	Claudin 3 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)