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Western blot analysis of various lysates, using Cleaved Caspase-7 antibody (CAB23154) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

Cleaved Caspase-7 Monoclonal Antibody (CAB23154)

Datasheet MSDS
SKU CAB23154
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms MCH3, CMH-1, LICE2, CASP-7, ICE-LAP3, Cleaved Caspase-7
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

Cleaved Caspase-7 Monoclonal Antibody (CAB23154)

The Cleaved Caspase-7 Monoclonal Antibody (CAB23154) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed and validated for use in various applications, including Western blot and immunohistochemistry, specifically targets the cleaved form of caspase-7, allowing for precise detection and analysis in cell samples.Caspase-7 is a key player in the execution phase of apoptosis, where it cleaves specific cellular proteins to initiate cell death processes. Understanding the activity and regulation of caspase-7 is important for unraveling the mechanisms underlying cell death pathways, with implications for cancer research, neurodegenerative diseases, and other physiological processes.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:Cleaved Caspase-7 Monoclonal Antibody
SKU:CAB23154
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC58776
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:CFRS LGFD VIVY NDCS CAKM QDLL KKAS EEDH TNAA CFAC ILLS HGEE NVIY GKDG VTPI KDLT AHFR GDRC KTLL EKPK LFFI QACR GTEL DDGI QADS G
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:MCH3, CMH-1, LICE2, CASP-7, ICE-LAP3, Cleaved Caspase-7
Positive Sample:Jurkat treated with Etoposide
Cellular Localization:Cytoplasm.
Calculated MW:34kDa
Observed MW:20kDa

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification MethodAffinity purification
Gene ID840
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells using Cleaved Caspase-7 Rabbit mAb (CAB23154) at 1:1000 dilution incubated overnight at 4℃. Jurkat cells were treated with Etoposide (25 μM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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