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Anti-CLPX Antibody (CAB17088)

SKU:
CAB17088
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
€129 - €329
Frequently bought together:

Description

Antibody Name:Anti-CLPX Antibody (CAB17088)
Antibody SKU:CAB17088
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human CLPX (NP_006651.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver, Rat live
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human CLPX (NP_006651.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LPVV VPLH SLDE KTLV QILT EPRN AVIP QYQA LFSM DKCE LNVT EDAL KAIA RLAL ERKT GARG LRSI MEKL LLEP MFEV PNSD IVCV EVDK EVVE GKKE P
Cellular Location:
Calculated MW:
Observed MW:69kDa
Synonyms:CLPX
Background:The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.
Western blot analysis of extracts of various cell lines, using CLPX antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Western blot analysis of extracts of various cell lines, using CLPX antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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