Anti-Complement factor D Antibody (CAB8117)

Antibody Name:	CFD Rabbit Polyclonal Antibody
Antibody SKU:	CAB8117
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human CFD (NP_001919.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A-549, 22Rv1, THP-1, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human CFD (NP_001919.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ILGG REAE AHAR PYMA SVQL NGAH LCGG VLVA EQWV LSAA HCLE DAAD GKVQ VLLG AHSL SQPE PSKR LYDV LRAV PHPD SQPD TIDH DLLL LQLS EKAT LGPA VRPL PWQR VDRD VAPG TLCD VAGW GIVN HAGR RPDS LQHV LLPV LDRA TCNR RTHH DGAI TERL MCAE SNRR DSCK GDSG GPLV CGGV LEGV VTSG SRVC GNRK KPGI YTRV ASYA AWID SVLA
Gene ID:	1675
Uniprot:	P00746
Cellular Location:	Secreted
Calculated MW:	27kDa
Observed MW:	27kDa

Synonyms:

CFD, ADIPSIN, ADN, DF, PFD

Background:

This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.

UniProt Protein Function:	CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.
UniProt Protein Details:	Protein type:Secreted; EC 3.4.21.46; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: extracellular region Molecular Function:serine-type endopeptidase activity; serine-type peptidase activity Biological Process: complement activation; complement activation, alternative pathway; platelet degranulation Disease: Complement Factor D Deficiency
NCBI Summary:	This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease. [provided by RefSeq, Nov 2015]
UniProt Code:	P00746
NCBI GenInfo Identifier:	158515408
NCBI Gene ID:	1675
NCBI Accession:	P00746.5
UniProt Secondary Accession:	P00746,Q5U5S1, Q86VJ5, Q8N4E0, Q8WZB4, B4DV76,
UniProt Related Accession:	P00746
Molecular Weight:	27,033 Da
NCBI Full Name:	Complement factor D
NCBI Synonym Full Names:	complement factor D
NCBI Official Symbol:	CFD
NCBI Official Synonym Symbols:	DF; ADN; PFD; ADIPSIN
NCBI Protein Information:	complement factor D
UniProt Protein Name:	Complement factor D
UniProt Synonym Protein Names:	Adipsin; C3 convertase activator; Properdin factor D
Protein Family:	Complement factor
UniProt Gene Name:	CFD
UniProt Entry Name:	CFAD_HUMAN

	Western blot analysis of extracts of various cell lines, using CFD antibody (CAB8117) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded human prostate using CFD antibody (CAB8117) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using CFD antibody (CAB8117) at dilution of 1:100 (40x lens).

ELISA Kits

Human Complement factor D / Factor D ELISA Kit

Human CFD (Complement Factor D) CLIA Kit (HUES00040)

Human Complement factor D (CFD) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)