Anti-CYP11B2 Antibody (CAB1443)

Antibody Name:	Anti-CYP11B2 Antibody
Antibody SKU:	CAB1443
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B2 (NP_000489.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, MCF7, 293T, Mouse brain, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 234-503 of human CYP11B2 (NP_000489.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TVQL MFMP RSLS RWIS PKVW KEHF EAWD CIFQ YGDN CIQK IYQE LAFN RPQH YTGI VAEL LLKA ELSL EAIK ANSM ELTA GSVD TTAF PLLM TLFE LARN PDVQ QILR QESL AAAA SISE HPQK ATTE LPLL RAAL KETL RLYP VGLF LERV VSSD LVLQ NYHI PAGT LVQV FLYS LGRN AALF PRPE RYNP QRWL DIRG SGRN FHHV PFGF GMRQ CLGR RLAE AEML LLLH HVLK HFLV ETLT QEDI KMVY SFIL RPGT SPLL TFRA IN
Gene ID:	1585
Uniprot:	P19099
Cellular Location:	Mitochondrion membrane
Calculated MW:	57kDa
Observed MW:	50kDa

Synonyms:

CYP11B2, ALDOS, CPN2, CYP11B, CYP11BL, CYPXIB2, P-450C18, P450C18, P450aldo

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency.

UniProt Protein Function:	CYP11B2: Preferentially catalyzes the conversion of 11- deoxycorticosterone to aldosterone via corticosterone and 18- hydroxycorticosterone. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 1 deficiency (CMO-1 deficiency); also known as aldosterone deficiency due to defect in 18- hydroxylase or aldosterone deficiency I. CMO-1 deficiency is an autosomal recessive disorder of aldosterone biosynthesis. There are two biochemically different forms of selective aldosterone deficiency be termed corticosterone methyloxidase (CMO) deficiency type 1 and type 2. In CMO-1 deficiency, aldosterone is undetectable in plasma, while its immediate precursor, 18- hydroxycorticosterone, is low or normal. Defects in CYP11B2 are the cause of corticosterone methyloxidase type 2 deficiency (CMO-2 deficiency). CMO-2 is an autosomal recessive disorder of aldosterone biosynthesis. In CMO-2 deficiency, aldosterone can be low or normal, but at the expense of increased secretion of 18- hydroxycorticosterone. Consequently, patients have a greatly increased ratio of 18-hydroxycorticosterone to aldosterone and a low ratio of corticosterone to 18-hydroxycorticosterone in serum. Defects in CYP11B2 are a cause of familial hyperaldosteronism type 1 (FH1). It is a disorder characterized by hypertension, variable hyperaldosteronism, and abnormal adrenal steroid production, including 18-oxocortisol and 18-hydroxycortisol. There is significant phenotypic heterogeneity, and some individuals never develop hypertension. The molecular defect causing hyperaldosteronism familial type 1 is an anti-Lepore-type fusion of the CYP11B1 and CYP11B2 genes. The hybrid gene has the promoting part of CYP11B1, ACTH-sensitive, and the coding part of CYP11B2. Belongs to the cytochrome P450 family.
UniProt Protein Details:	Protein type:Lipid Metabolism - C21-steroid hormone; EC 1.14.15.5; Oxidoreductase; EC 1.14.15.4; Lipid Metabolism - androgen and estrogen; Mitochondrial Chromosomal Location of Human Ortholog: 8q21-q22 Cellular Component: mitochondrial inner membrane; mitochondrion Molecular Function:corticosterone 18-monooxygenase activity; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; steroid 11-beta-monooxygenase activity; steroid hydroxylase activity Biological Process: aldosterone biosynthetic process; aldosterone mediated regulation of blood volume; C21-steroid hormone biosynthetic process; cellular response to hormone stimulus; cholesterol metabolic process; mineralocorticoid biosynthetic process; potassium ion homeostasis; renal water homeostasis; sodium ion homeostasis; sterol metabolic process Disease: Corticosterone Methyloxidase Type I Deficiency; Corticosterone Methyloxidase Type Ii Deficiency
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:	P19099
NCBI GenInfo Identifier:	3041666
NCBI Gene ID:	1585
NCBI Accession:	P19099.3
UniProt Secondary Accession:	P19099,Q16726, B0ZBE4,
UniProt Related Accession:	P19099
Molecular Weight:	57,560 Da
NCBI Full Name:	Cytochrome P450 11B2, mitochondrial
NCBI Synonym Full Names:	cytochrome P450 family 11 subfamily B member 2
NCBI Official Symbol:	CYP11B2
NCBI Official Synonym Symbols:	CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo
NCBI Protein Information:	cytochrome P450 11B2, mitochondrial
UniProt Protein Name:	Cytochrome P450 11B2, mitochondrial
UniProt Synonym Protein Names:	Aldosterone synthase (EC:1.14.15.4, EC:1.14.15.5); ALDOS; Aldosterone-synthesizing enzyme; CYPXIB2; Cytochrome P-450Aldo; Cytochrome P-450C18; Steroid 18-hydroxylase
Protein Family:	Cytochrome
UniProt Gene Name:	CYP11B2
UniProt Entry Name:	C11B2_HUMAN

	Western blot analysis of extracts of various cell lines, using CYP11B2 antibody (CAB1443) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded rat adrenal gland using CYP11B2 antibody (CAB1443) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse adrenal gland using CYP11B2 antibody (CAB1443) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)