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Anti-CYP1B1 Antibody (CAB13967)

SKU:
CAB13967
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CYP1B1 Antibody
Antibody SKU:CAB13967
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human CYP1B1 (NP_000095.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human CYP1B1 (NP_000095.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPWL QYFP NPVR TVFR EFEQ LNRN FSNF ILDK FLRH CESL RPGA APRD MMDA FILS AEKK AAGD SHGG GARL DLEN VPAT ITDI FGAS QDTL STAL QWLL LLFT RYPD VQTR VQAE LDQV VGRD RLPC MGDQ PNLP YVLA FLYE AMRF SSFV PVTI PHAT TANT SVLG YHIP KDTV VFVN QWSV NHDP LKWP NPEN FDPA RFLD KDGL INKD LTSR VMIF SVGK RRCI GEEL SKMQ LFLF ISIL AHQC DFRA NPNE PAKM NFSY GLTI KPKS FKVN VTLR ESME LLDS AVQN LQAK ETCQ
Gene ID:1545
Uniprot:Q16678
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion, Peripheral membrane protein
Calculated MW:60kDa
Observed MW:Refer to figures
Synonyms:CYP1B1, ASGD6, CP1B, CYPIB1, GLC3A, P4501B1
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.
UniProt Protein Function:CYP1B1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A). GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG). POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes. Defects in CYP1B1 are a cause of Peters anomaly (PAN). Peters anomaly is a congenital defect of the anterior chamber of the eye. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 2p22.2

Cellular Component: endoplasmic reticulum membrane; mitochondrion

Molecular Function:iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; estrogen metabolic process; collagen fibril organization; retinal metabolic process; positive regulation of apoptosis; response to toxin; positive regulation of JAK-STAT cascade; negative regulation of cell proliferation; visual perception; retinol metabolic process; arachidonic acid metabolic process; angiogenesis; nitric oxide biosynthetic process; cell adhesion; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; epoxygenase P450 pathway; positive regulation of angiogenesis; inhibition of NF-kappaB transcription factor; xenobiotic metabolic process; toxin metabolic process; endothelial cell migration; blood vessel morphogenesis; aromatic compound metabolic process; membrane lipid catabolic process; induction of apoptosis by oxidative stress; sterol metabolic process

Disease: Peters Anomaly; Glaucoma 3, Primary Congenital, A; Glaucoma 3, Primary Infantile, B

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]
UniProt Code:Q16678
NCBI GenInfo Identifier:48429256
NCBI Gene ID:1545
NCBI Accession:Q16678.2
UniProt Secondary Accession:Q16678,Q5TZW8, Q93089, Q9H316,
UniProt Related Accession:Q16678
Molecular Weight:
NCBI Full Name:Cytochrome P450 1B1
NCBI Synonym Full Names:cytochrome P450, family 1, subfamily B, polypeptide 1
NCBI Official Symbol:CYP1B1  
NCBI Official Synonym Symbols:CP1B; GLC3A; CYPIB1; P4501B1  
NCBI Protein Information:cytochrome P450 1B1; microsomal monooxygenase; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile)
UniProt Protein Name:Cytochrome P450 1B1
UniProt Synonym Protein Names:CYPIB1
Protein Family:Cytochrome
UniProt Gene Name:CYP1B1  
UniProt Entry Name:CP1B1_HUMAN
Anti-CYP1B1 Antibody (CAB13967)Immunofluorescence analysis of HeLa cells using CYP1B1 antibody (CAB13967) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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