Western blot analysis of various lysates, using CYP27A1 Rabbit mAb (CAB23250) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The CYP27A1 Monoclonal Antibody (CAB23250) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using advanced monoclonal antibody technology, specifically targets the CYP27A1 protein and is highly reactive with human samples.CYP27A1 plays a crucial role in maintaining cholesterol homeostasis and bile acid synthesis, making it a key player in lipid metabolism. Dysregulation of CYP27A1 activity has been linked to various metabolic disorders, including atherosclerosis and gallstone formation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
CYP27A1 Monoclonal Antibody
SKU:
CAB23250
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC59606
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CTX, CP27, CYP27, CYP27A1
Positive Sample:
Mouse liver, Rat liver
Cellular Localization:
Mitochondrion Membrane.
Calculated MW:
60kDa
Observed MW:
55kDa
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.
Purification Method
Affinity purification
Gene ID
1593
RRID
AB_3674587
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CYP27A1 Rabbit mAb (CAB23250) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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