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Anti-CYP27A1 Antibody (CAB1982)

SKU:
CAB1982
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-CYP27A1 Antibody CAB1982
  • Metabolism Antibodies 2 Anti-CYP27A1 Antibody CAB1982
€139 - €419
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Description

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Antibody Name:Anti-CYP27A1 Antibody
Antibody SKU:CAB1982
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human CYP27A1 (NP_000775.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, Mouse kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 200-400 of human CYP27A1 (NP_000775.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NQVS DMAQ LFYY FALE AICY ILFE KRIG CLQR SIPE DTVT FVRS IGLM FQNS LYAT FLPK WTRP VLPF WKRY LDGW NAIF SFGK KLID EKLE DMEA QLQA AGPD GIQV SGYL HFLL ASGQ LSPR EAMG SLPE LLMA GVDT TSNT LTWA LYHL SKDP EIQE ALHE EVVG VVPA GQVP QHKD FAHM PLLK AVLK ETLR LYPV V
Gene ID:1593
Uniprot:Q02318
Cellular Location:Mitochondrion membrane
Calculated MW:60kDa
Observed MW:60kDa
Synonyms:CYP27A1, CP27, CTX, CYP27
Background:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.
UniProt Protein Function:CYP27A1: Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity. Defects in CYP27A1 are the cause of cerebrotendinous xanthomatosis (CTX). CTX is a rare sterol storage disorder characterized clinically by progressive neurologic dysfunction, premature atherosclerosis, and cataracts. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:Oxidoreductase; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.15; Mitochondrial

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function:cholestanetriol 26-monooxygenase activity; iron ion binding; cholesterol 26-hydroxylase activity; heme binding; steroid hydroxylase activity; vitamin D3 25-hydroxylase activity

Biological Process: bile acid biosynthetic process; cholesterol metabolic process; bile acid metabolic process; xenobiotic metabolic process; sterol metabolic process

Disease: Cerebrotendinous Xanthomatosis

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]
UniProt Code:Q02318
NCBI GenInfo Identifier:4503211
NCBI Gene ID:1593
NCBI Accession:NP_000775.1
UniProt Related Accession:Q02318
Molecular Weight:Predicted Molecular Mass: 39kDaAccurate Molecular Mass: 43kDa
NCBI Full Name:sterol 26-hydroxylase, mitochondrial
NCBI Synonym Full Names:cytochrome P450 family 27 subfamily A member 1
NCBI Official Symbol:CYP27A1  
NCBI Official Synonym Symbols:CTX; CP27; CYP27  
NCBI Protein Information:sterol 26-hydroxylase, mitochondrial
UniProt Protein Name:Sterol 26-hydroxylase, mitochondrial
UniProt Synonym Protein Names:5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase
Protein Family:FK506-binding protein
UniProt Gene Name:CYP27A1  
UniProt Entry Name:CP27A_HUMAN
Anti-CYP27A1 Antibody (CAB1982)Western blot analysis of extracts of various cell lines, using CYP27A1 antibody (CAB1982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-CYP27A1 Antibody (CAB1982)Immunofluorescence analysis of HeLa cells using CYP27A1 antibody (CAB1982) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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