Anti-DBN1 Antibody (CAB6366)

Antibody Name:	Anti-DBN1 Antibody
Antibody SKU:	CAB6366
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-649 of human DBN1 (NP_004386.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	BT-474, HeLa, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 350-649 of human DBN1 (NP_004386.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EQIE RALD EVTS SQPP PLPP PPPP AQET QEPS PILD SEET RAAA PQAW AGPM EEPP QAQA PPRG PGSP AEDL MFME SAEQ AVLA APVE PATA DATE IHDA ADTI ETDT ATAD TTVA NNVP PAAT SLID LWPG NGEG ASTL QGEP RAPT PPSG TEVT LAEV PLLD EVAP EPLL PAGE GCAT LLNF DELP EPPA TFCD PEEV EGEP LAAP QTPT LPSA LEEL EQEQ EPEP HLLT NGET TQKE GTQA SEGY FSQS QEEE FAQS EELC AKAP PPVF YNKP PEID ITCW DADP VPEE EEGF EGGD
Gene ID:	1627
Uniprot:	Q16643
Cellular Location:	Cell junction, Cell projection, Cytoplasm, cell cortex, growth cone
Calculated MW:	71kDa/76kDa
Observed MW:	110kDa

Synonyms:

DBN1, D0S117E, drebrin

Background:

The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene.

UniProt Protein Function:	DBN1: a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. Expressed in brain neurons, heart, placenta, skeletal muscle, kidney and pancreas. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: actin cytoskeleton; growth cone; plasma membrane Molecular Function:profilin binding; protein binding Biological Process: actin filament organization
NCBI Summary:	The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16643
NCBI GenInfo Identifier:	215274247
NCBI Gene ID:	1627
NCBI Accession:	Q16643.4
UniProt Secondary Accession:	Q16643,Q9UFZ5, A8MV58, B2RBG0,
UniProt Related Accession:	Q16643
Molecular Weight:	76,300 Da
NCBI Full Name:	Drebrin
NCBI Synonym Full Names:	drebrin 1
NCBI Official Symbol:	DBN1
NCBI Official Synonym Symbols:	D0S117E
NCBI Protein Information:	drebrin
UniProt Protein Name:	Drebrin
UniProt Synonym Protein Names:	Developmentally-regulated brain protein
Protein Family:	Drebrin
UniProt Gene Name:	DBN1
UniProt Entry Name:	DREB_HUMAN

	Western blot analysis of extracts of various cell lines, using DBN1 antibody (CAB6366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using DBN1 antibody (CAB6366). Blue: DAPI for nuclear staining.

Antibodies

DBN1 Antibody (PACO08751)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)