E.Coli GLDA E.coli Recombinant Protein (RPPB1693)
- Product type:
- Recombinant Protein
- GLDA E.coli
- Glycerol dehydrogenase
- Escherichia Coli
|Product Name:||E.Coli GLDA E.coli Recombinant Protein|
|Synonyms:||ECK3937,JW5556, Glycerol dehydrogenase, GDH, GLDH, b3945.|
|Physical Appearance:||Sterile Filtered colorless solution.|
|Formulation:||GLDA protein solution (1mg/ml) containing Phosphatebuffered saline (pH7.4) and 10% glycerol.|
|Stability:||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.|
|Purity:||Greater than 95.0% as determined by SDS-PAGE.|
|Amino Acid Sequence:||MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQSTVEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTIASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGATTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVHNGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAEAACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE|
|Biological Activity:||Specific activity: > 14 Units/ml. One unit willoxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C.|
Glycerol dehydrogenase (GldA) catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). The GldA protein allows microorganisms to use glycerol as a source of carbon under anaerobic conditions. Furthermore, in E.coli GldA has an imperative role by regulating the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. GldA possesses an extensive substrate specificity, due to its ability to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively.
GLDA E.coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 390 amino acids (1-367 a.a) and having a molecular mass of 41.1kDa. GLDA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.