Immunohistochemistry analysis of paraffin-embedded rat spleen using ECE1 antibody (CAB21824) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
The ECE1 Polyclonal Antibody (CAB21824) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the ECE1 enzyme, this antibody allows for the detection and analysis of ECE1 expression in various cell types, making it ideal for studies in cardiovascular research and hypertension.ECE1 plays a crucial role in the conversion of big endothelin to active endothelin, a process that has implications in the development of cardiovascular diseases such as hypertension and heart failure.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ECE1 Polyclonal Antibody
SKU:
CAB21824
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ECE, ECE1
Positive Sample:
HeLa
Cellular Localization:
Cell Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
87kDa
Observed MW:
125-135kDa
The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method
Affinity purification
Gene ID
1889
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using ECE1 Rabbit pAb (CAB21824) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:180s.
