Anti-EFNA1 Antibody (CAB5341)

Antibody Name:	Anti-EFNA1 Antibody
Antibody SKU:	CAB5341
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-182 of human EFNA1 (NP_004419.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	H460, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-182 of human EFNA1 (NP_004419.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DRHT VFWN SSNP KFRN EDYT IHVQ LNDY VDII CPHY EDHS VADA AMEQ YILY LVEH EEYQ LCQP QSKD QVRW QCNR PSAK HGPE KLSE KFQR FTPF TLGK EFKE GHSY YYIS KPIH QHED RCLR LKVT VSGK ITHS PQAH DNPQ EKRL AADD PEVR VLHS IGHS
Gene ID:	1942
Uniprot:	P20827
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor, Secreted
Calculated MW:	21kDa/23kDa
Observed MW:	21-23kDa

Synonyms:

EFNA1, B61, ECKLG, EFL1, EPLG1, LERK-1, LERK1, TNFAIP4, ephrin-A1

Background:

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.

UniProt Protein Function:	Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
NCBI Summary:	This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]
UniProt Code:	P20827
NCBI GenInfo Identifier:	73920206
NCBI Gene ID:	1942
NCBI Accession:	P20827.2
UniProt Secondary Accession:	P20827,Q5SR60, Q5SR61, Q6I9T9, Q8N578, D3DV86,
UniProt Related Accession:	P20827
Molecular Weight:	21,246 Da
NCBI Full Name:	Ephrin-A1
NCBI Synonym Full Names:	ephrin A1
NCBI Official Symbol:	EFNA1
NCBI Official Synonym Symbols:	B61; EFL1; ECKLG; EPLG1; LERK1; LERK-1; TNFAIP4
NCBI Protein Information:	ephrin-A1
UniProt Protein Name:	Ephrin-A1
UniProt Synonym Protein Names:	EPH-related receptor tyrosine kinase ligand 1; LERK-1
Protein Family:	Ephrin
UniProt Gene Name:	EFNA1

	Western blot analysis of extracts of various cell lines, using EFNA1 antibody (CAB5341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human prostate using EFNA1 Antibody (CAB5341) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using EFNA1 Antibody (CAB5341) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using EFNA1 Antibody (CAB5341) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
EFNA1 Antibody (PACO06467)	Human Ephrin A1 / EFNA1 ELISA Kit
	Human EFNA1 (Ephrin A1) CLIA Kit (HUES00528)
	Human EFNA1 (Ephrin A1) ELISA Kit (HUES02003)
	Human Ephrin-A1 (EFNA1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)