Western blot analysis of various lysates, using FH Rabbit pAb (CAB21403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The FH Polyclonal Antibody (CAB21403) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced by Assay Genie, is raised in rabbits and specifically designed for use in Western blot applications. It is highly reactive with human samples, enabling accurate detection and analysis of FH protein levels in various cell types.FH is a critical component of the complement system, playing a crucial role in regulating the immune response and protecting the body against infections. Dysregulation of FH has been implicated in various diseases, including age-related macular degeneration and atypical hemolytic uremic syndrome.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FH Polyclonal Antibody
SKU:
CAB21403
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MCL, FMRD, HsFH, LRCC, HLRCC, MCUL1, FH
Positive Sample:
293T, Hela, NIH/3T3, C2C12, Rat liver
Cellular Localization:
Cytoplasm, Mitochondrion.
Calculated MW:
55kDa
Observed MW:
49kDa
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
Purification Method
Affinity purification
Gene ID
2271
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using FH Rabbit pAb (CAB21403) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type(WT) and FH knockout (KO) 293T(KO) cells, using [KO Validated] FH Rabbit pAb (CAB21403) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
