Anti-Fibronectin Antibody (CAB7488)

Antibody Name:	Anti-Fibronectin Antibody
Antibody SKU:	CAB7488
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human Fibronectin (NP_002017.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human Fibronectin (NP_002017.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLRG PGPG LLLL AVQC LGTA VPST GASK SKRQ AQQM VQPQ SPVA VSQS KPGC YDNG KHYQ INQQ WERT YLGN ALVC TCYG GSRG FNCE SKPE AEET CFDK YTGN TYRV GDTY ERPK DSMI WDCT CIGA
Gene ID:	2335
Uniprot:	P02751
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	71-73kDa/221-272kDa
Observed MW:	290kDa

Synonyms:

FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, fibronectin

Background:

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.

UniProt Protein Function:	FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell adhesion; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q34 Cellular Component: extracellular matrix; extracellular space; fibrinogen complex; apical plasma membrane; extracellular region; ER-Golgi intermediate compartment; basal lamina Molecular Function:heparin binding; collagen binding; integrin binding; protein binding; protease activator activity; protease binding Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; response to wounding; acute-phase response; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; peptide cross-linking; angiogenesis; cell adhesion; blood coagulation; leukocyte migration Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency
NCBI Summary:	This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	P02751
NCBI GenInfo Identifier:	300669710
NCBI Gene ID:	2335
NCBI Accession:	P02751.4
UniProt Secondary Accession:	P02751,O95609, O95610, Q14312, Q14325, Q14326, Q17RV7 Q564H7, Q585T2, B7ZLF0, E9PE77, E9PG29,
UniProt Related Accession:	P02751
Molecular Weight:	256,502 Da
NCBI Full Name:	Fibronectin
NCBI Synonym Full Names:	fibronectin 1
NCBI Official Symbol:	FN1
NCBI Official Synonym Symbols:	FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
NCBI Protein Information:	fibronectin; cold-insoluble globulin; migration-stimulating factor
UniProt Protein Name:	Fibronectin
UniProt Synonym Protein Names:	Cold-insoluble globulin; CIG
Protein Family:	Fibronectin
UniProt Gene Name:	FN1
UniProt Entry Name:	FINC_HUMAN

	Western blot analysis of extracts of NIH/3T3 cells, using Fibronectin antibody (CAB7488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using Fibronectin Antibody (CAB7488) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using Fibronectin Antibody (CAB7488) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using Fibronectin Antibody (CAB7488) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Fibronectin Antibody (CAB12932)	Human FN (Fibronectin) CLIA Kit (HUES00153)
Anti-Fibronectin Antibody (CAB16678)	Human FN (Fibronectin) ELISA Kit (HUES01446)
FN1 Antibody (PACO07088)	Human Fibronectin (FN1) ELISA Kit
FN1 Antibody (PACO07484)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)