The FoxO1/FoxO3a/FoxO4 Antibody (CAB17978) is a high-quality antibody developed for reliable detection and analysis of target proteins. These proteins are critical regulators of various cellular processes, including cell cycle progression, apoptosis, and glucose metabolism. The antibody, generated in rabbits, shows high specificity for human samples and has been validated for use in Western blot applications.By targeting all three FOXO isoforms (FOXO1, FOXO3a, and FOXO4), this antibody allows for comprehensive analysis of FOXO protein expression and activity in different cell types and experimental conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FoxO1/FoxO3a/FoxO4 Antibody
SKU:
CAB17978
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The Forkhead family of transcription factors mediates many aspects of physiology, including stress response, metabolism, commitment to apoptosis, and development. The Forkhead box subfamily O (FoxO) proteins have garnered particular interest due to their involvement in the modulation of cancer, metabolism and cardiovascular biology.In mammals, four subfamily members have been identified: FOXO1 (previously also known as FKHR), FOXO3 (also known as FKHRL1), FOXO4 (also known as AFX),and FOXO6.
Purification Method
Affinity purification
Gene ID
2308 2309 4303
RRID
AB_2861779
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FOXO1/FOXO3A/FOXO4 Rabbit pAb (CAB17978) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using FOXO1/FOXO3A/FOXO4 Rabbit pAb (CAB17978) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis using FOXO1/FOXO3A/FOXO4 Rabbit pAb (CAB17978) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
