FTO Rabbit Monoclonal Antibody (CAB3861)

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CAB3861
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Description

Technical Manual

FTO Rabbit Monoclonal Antibody

Product Name:FTO Rabbit Monoclonal Antibody
Product SKU:CAB3861
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]

Immunogen:A synthesized peptide derived from human FTO
Sequence:

Email for sequence

Gene ID:79068
Swiss Prot:Q9C0B1
Synonyms:ALKBH9; BMIQ14; GDFD
Calculated MW:58kDa
Observed MW:60KDa
Reactivity:Human
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:HeLa, 293T, SH-SY5Y
Cellular Location:

Product Images

FTO Rabbit Monoclonal Antibody (CAB3861)

Western blot - FTO Rabbit mAb (CAB3861)

Uniprot Information

UniProt Protein Function:FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.14.11.-

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nuclear speck; nucleus

Molecular Function:ferrous iron binding; DNA-N1-methyladenine dioxygenase activity

Biological Process: DNA dealkylation; RNA repair; regulation of multicellular organism growth; thermoregulation

Disease: Growth Retardation, Developmental Delay, Coarse Facies, And Early Death

NCBI Summary:This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
UniProt Code:Q9C0B1
NCBI GenInfo Identifier:148841515
NCBI Gene ID:79068
NCBI Accession:Q9C0B1.3
UniProt Secondary Accession:Q9C0B1,Q0P676, Q7Z785, A2RUH1, B2RNS0,
UniProt Related Accession:Q9C0B1
Molecular Weight:12,218 Da
NCBI Full Name:Alpha-ketoglutarate-dependent dioxygenase FTO
NCBI Synonym Full Names:fat mass and obesity associated
NCBI Official Symbol:FTO 
NCBI Official Synonym Symbols:ALKBH9 
NCBI Protein Information:alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; AlkB homolog 9; fat mass and obesity-associated protein
UniProt Protein Name:Alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Synonym Protein Names:Fat mass and obesity-associated protein
Protein Family:Alpha-ketoglutarate-dependent dioxygenase
UniProt Gene Name:FTO 
UniProt Entry Name:FTO_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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