Anti-PAEP Antibody (CAB5751)

Antibody Name:	Anti-PAEP Antibody
Antibody SKU:	CAB5751
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-180 of human PAEP (NP_002562.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human
Positive Samples:	U-937, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-180 of human PAEP (NP_002562.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDIP QTKQ DLEL PKLA GTWH SMAM ATNN ISLM ATLK APLR VHIT SLLP TPED NLEI VLHR WENN SCVE KKVL GEKT ENPK KFKI NYTV ANEA TLLD TDYD NFLF LCLQ DTTT PIQS MMCQ YLAR VLVE DDEI MQGF IRAF RPLP RHLW YLLD LKQM EEPC RF
Gene ID:	5047
Uniprot:	P09466
Cellular Location:	Secreted
Calculated MW:	9kDa/18kDa/20kDa
Observed MW:	26kDa

Synonyms:

PAEP, GD, GdA, GdF, GdS, PAEG, PEP, PP14

Background:

This gene is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct and essential roles in regulating a uterine environment suitable for pregnancy and in the timing and occurrence of the appropriate sequence of events in the fertilization process. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	PAEP: This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy. Belongs to the calycin superfamily. Lipocalin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 9q34.3 Molecular Function:protein binding Biological Process: apoptosis; multicellular organismal development; positive regulation of granulocyte macrophage colony-stimulating factor production
NCBI Summary:	This gene is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct and essential roles in regulating a uterine environment suitable for pregnancy and in the timing and occurrence of the appropriate sequence of events in the fertilization process. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Code:	P09466
NCBI GenInfo Identifier:	130701
NCBI Gene ID:	5047
NCBI Accession:	P09466.2
UniProt Secondary Accession:	P09466,Q5T6T1, Q9UG92,
UniProt Related Accession:	P09466
Molecular Weight:	21kDa
NCBI Full Name:	Glycodelin
NCBI Synonym Full Names:	progestagen associated endometrial protein
NCBI Official Symbol:	PAEP
NCBI Official Synonym Symbols:	GD; GdA; GdF; GdS; PEP; PAEG; PP14
NCBI Protein Information:	glycodelin
UniProt Protein Name:	Glycodelin
UniProt Synonym Protein Names:	Placental protein 14; PP14; Pregnancy-associated endometrial alpha-2 globulin; PAEG; PEG; Progestagen-associated endometrial protein; Progesterone-associated endometrial protein
Protein Family:	Glycodelin
UniProt Gene Name:	PAEP

	Western blot analysis of extracts of various cell lines, using PAEP antibody (CAB5751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using PAEP antibody (CAB5751). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PAEP Antibody (CAB14757)	Human Glycodelin / Progestagen-Associated Endometrial Protein ELISA Kit
	Human Glycodelin (PAEP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)