The GPD2 Antibody (CAB16378) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the GPD2 protein, this antibody enables precise detection and analysis of GPD2 expression in a variety of cell types, making it ideal for investigations in metabolism, lipid biology, and related fields.GPD2 plays a crucial role in cellular energy metabolism and lipid metabolism, making it a key player in processes like glycerol utilization, lipid storage, and energy production.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GPD2 Antibody
SKU:
CAB16378
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GDH2, GPDM, mGDH, mGPDH, GPD2
Positive Sample:
U-87 MG, HeLa, MCF7
Cellular Localization:
Mitochondrion.
Calculated MW:
81kDa
Observed MW:
74kDa
The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
2820
RRID
AB_2769657
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using GPD2 Rabbit pAb (CAB16378) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
