Anti-GPI Antibody (CAB13308)
- SKU:
- CAB13308
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-GPI Antibody |
| Antibody SKU: | CAB13308 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human GPI (NP_000166.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, BT-474, MCF7, HeLa, Mouse kidney, Mouse heart, Mouse brain, Rat brain, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human GPI (NP_000166.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAL TRDP QFQK LQQW YREH RSEL NLRR LFDA NKDR FNHF SLTL NTNH GHIL VDYS KNLV TEDV MRML VDLA KSRG VEAA RERM FNGE KINY TEGR AVLH VALR NRSN TPIL VDGK DVMP EVNK VLDK MKSF CQRV RSGD WKGY TGKT ITDV INIG IGGS DLGP LMVT EALK PYSS GGPR VWYV SNID GTHI AKTL AQLN PESS LFII ASKT FTTQ ETIT NAET AKEW FLQA AKDP SAVA KHFV ALST NTTK VKEF GIDP QNMF EFWD WVGG RYSL WSAI |
| Gene ID: | 2821 |
| Uniprot: | P06744 |
| Cellular Location: | Cytoplasm, Secreted |
| Calculated MW: | 63kDa/64kDa |
| Observed MW: | 56kDa |
| Synonyms: | GPI, AMF, GNPI, NLK, PGI, PHI, SA-36, SA36 |
| Background: | This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. |
| UniProt Protein Details: | Protein type:Isomerase; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 5.3.1.9; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cytokine; Carbohydrate Metabolism - pentose phosphate pathway; Apoptosis Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol Molecular Function:monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; glycolysis; glucose 6-phosphate metabolic process; glucose metabolic process; pathogenesis; negative regulation of caspase activity; humoral immune response; gluconeogenesis; learning and/or memory; hemostasis; carbohydrate metabolic process; negative regulation of neuron apoptosis; angiogenesis Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency |
| NCBI Summary: | This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | P06744 |
| NCBI GenInfo Identifier: | 17380385 |
| NCBI Gene ID: | 2821 |
| NCBI Accession: | P06744.4 |
| UniProt Secondary Accession: | P06744,Q9BRD3, Q9BSK5, Q9UHE6, B4DG39, |
| UniProt Related Accession: | P06744 |
| Molecular Weight: | 558 |
| NCBI Full Name: | Glucose-6-phosphate isomerase |
| NCBI Synonym Full Names: | glucose-6-phosphate isomerase |
| NCBI Official Symbol: | GPIÂ Â |
| NCBI Official Synonym Symbols: | AMF; NLK; PGI; PHI; GNPI; SA36; SA-36Â Â |
| NCBI Protein Information: | glucose-6-phosphate isomerase; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; phosphohexose isomerase; phosphoglucose isomerase; autocrine motility factor; hexosephosphate isomerase; glucose phosph |
| UniProt Protein Name: | Glucose-6-phosphate isomerase |
| UniProt Synonym Protein Names: | Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36 |
| Protein Family: | Glucose-6-phosphate isomerase |
| UniProt Gene Name: | GPIÂ Â |
| UniProt Entry Name: | G6PI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using GPI antibody (CAB13308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using GPI antibody (CAB13308) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using GPI antibody (CAB13308) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using GPI antibody (CAB13308) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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