Western blot analysis of extracts of various cell lines, using GPRC5B antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
The GPRC5B Monoclonal Antibody (CAB20946) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in mice, is highly specific for human GPRC5B and has been validated for use in immunohistochemistry applications. By targeting the GPRC5B protein, this antibody allows for the detection and analysis of GPRC5B expression in different tissues and cell types, making it an essential tool for investigations in cell biology and cancer research.GPRC5B, also known as retinoic acid-inducible protein 3 (RAI3), is involved in retinoic acid signaling pathways and has been linked to the development and progression of certain types of cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
GPRC5B Monoclonal Antibody
SKU:
CAB20946
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2932
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
51704
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GPRC5B Rabbit mAb (CAB20946) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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