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Anti-HAPLN1 Antibody (CAB6616)

SKU:
CAB6616
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 10 Anti-HAPLN1 Antibody CAB6616
  • Cell Biology Antibodies 10 Anti-HAPLN1 Antibody CAB6616
  • Cell Biology Antibodies 10 Anti-HAPLN1 Antibody CAB6616
  • Cell Biology Antibodies 10 Anti-HAPLN1 Antibody CAB6616
€139 - €419
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Description

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Antibody Name:Anti-HAPLN1 Antibody
Antibody SKU:CAB6616
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 165-354 of human HAPLN1 (NP_001875.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Mouse
Positive Samples:HeLa, A375, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 165-354 of human HAPLN1 (NP_001875.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PRLG RYNL NFHE AQQA CLDQ DAVI ASFD QLYD AWRG GLDW CNAG WLSD GSVQ YPIT KPRE PCGG QNTV PGVR NYGF WDKD KSRY DVFC FTSN FNGR FYYL IHPT KLTY DEAV QACL NDGA QIAK VGQI FAAW KILG YDRC DAGW LADG SVRY PISR PRRR CSPT EAAV RFVG FPDK KHKL YGVY CFRA YN
Gene ID:1404
Uniprot:P10915
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:40kDa
Observed MW:50kDa
Synonyms:HAPLN1, CRT1, CRTL1
Background:
UniProt Protein Function:HAPLN1: Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix. Belongs to the HAPLN family.
UniProt Protein Details:

Protein type:Extracellular matrix

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function:extracellular matrix structural constituent; hyaluronic acid binding

Biological Process: central nervous system development; extracellular matrix organization and biogenesis; skeletal development

UniProt Code:P10915
NCBI GenInfo Identifier:130310
NCBI Gene ID:1404
NCBI Accession:P10915.2
UniProt Secondary Accession:P10915,B2R9A9,
UniProt Related Accession:P10915
Molecular Weight:40kDa
NCBI Full Name:Hyaluronan and proteoglycan link protein 1
NCBI Synonym Full Names:hyaluronan and proteoglycan link protein 1
NCBI Official Symbol:HAPLN1  
NCBI Official Synonym Symbols:CRT1; CRTL1  
NCBI Protein Information:hyaluronan and proteoglycan link protein 1
UniProt Protein Name:Hyaluronan and proteoglycan link protein 1
UniProt Synonym Protein Names:Cartilage-linking protein 1; Cartilage-link protein; Proteoglycan link protein
Protein Family:Hyaluronan and proteoglycan link protein
UniProt Gene Name:HAPLN1  
Anti-HAPLN1 Antibody (CAB6616)Western blot analysis of extracts of various cell lines, using HAPLN1 antibody (CAB6616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-HAPLN1 Antibody (CAB6616)Immunohistochemistry of paraffin-embedded human liver cancer using HAPLN1 antibody (CAB6616) at dilution of 1:100 (40x lens).
Anti-HAPLN1 Antibody (CAB6616)Immunohistochemistry of paraffin-embedded human stomach using HAPLN1 antibody (CAB6616) at dilution of 1:100 (40x lens).
Anti-HAPLN1 Antibody (CAB6616)Immunofluorescence analysis of A549 cells using HAPLN1 antibody (CAB6616). Blue: DAPI for nuclear staining.

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