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Anti-HDAC1 Mouse Monoclonal Antibody (CAB18304)

SKU:
CAB18304
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Mouse
Isotype:
IgG
Research Area:
Cell Biology
€139 - €329
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-HDAC1 Mouse Monoclonal Antibody
Antibody SKU:CAB18304
Antibody Size:20uL, 50uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Mouse
Immunogen:Recombinant protein of human HDAC1.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, 293T, BxPC-3
Immunogen:Recombinant protein of human HDAC1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:62kDa
Western blot - HDAC1 Mouse mAb Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - HDAC1 Mouse mAb Immunohistochemistry of paraffin-embedded Human esophageal using HDAC1 Mouse mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
Chromatin Immunoprecipitation - HDAC1 Mouse mAb Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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