Anti-HLA-A Antibody (CAB2167)

Antibody Name:	Anti-HLA-A Antibody
Antibody SKU:	CAB2167
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human HLA-A (NP_001229687.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, DU145, SW480, HepG2, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human HLA-A (NP_001229687.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SVSR PGRG EPRF IAVG YVDD TQFV RFDS DAAS QKME PRAP WIEQ EGPE YWDQ ETRN MKAH SQTD RANL GTLR GYYN QSED GSHT IQIM YGCD VGPD GRFL RGYR QDAY DGKD YIAL NEDL RSWT AADM AAQI TKRK WEAV HAAE QRRV YLEG RCVD GLRR YLEN GKET LQRT DPPK THMT HHPI SDHE ATLR CWAL GFYP AEIT LTWQ RDGE DQTQ DTEL VETR PAGD GTFQ KWAA VVVP SGEE QRYT CHV
Gene ID:	3105
Uniprot:	P04439/P30443
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	40kDa
Observed MW:	43kDa

Synonyms:

HLAA, HLA-A

Background:

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.

UniProt Protein Function:	Involved in the presentation of foreign antigens to the immune system.
NCBI Summary:	HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P04439
NCBI GenInfo Identifier:	13124681
NCBI Gene ID:	3105
NCBI Accession:	P01891.4
UniProt Secondary Accession:	P04439,O19673, O19695, O19794, O19795, O43907, O77938 O98010, P10315, P79505, Q9MYA5, Q9MYC4,
UniProt Related Accession:	P01891,P01892,P04439,P05534,P10314,P10316,P13746,P16188,P16189,P16190
Molecular Weight:	40,909 Da
NCBI Full Name:	HLA class I histocompatibility antigen, A-68 alpha chain
NCBI Synonym Full Names:	major histocompatibility complex, class I, A
NCBI Official Symbol:	HLA-A
NCBI Official Synonym Symbols:	HLAA
NCBI Protein Information:	HLA class I histocompatibility antigen, A-1 alpha chain; LOW QUALITY PROTEIN: HLA class I histocompatibility antigen, A-1 alpha chain
UniProt Protein Name:	HLA class I histocompatibility antigen, A-68 alpha chain
UniProt Synonym Protein Names:	Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68
Protein Family:	HLA class I histocompatibility antigen
UniProt Gene Name:	HLA-A

	Western blot analysis of extracts of various cell lines, using HLA-A antibody (CAB2167) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human liver damage using HLA-A antibody (CAB2167) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using HLA-A antibody (CAB2167) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using HLA-A antibody (CAB2167) at dilution of 1:100 (40x lens).

Antibodies

Anti-HLA-A Antibody (CAB17494)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)