Anti-HLF Antibody (CAB3143)

Antibody Name:	Anti-HLF Antibody
Antibody SKU:	CAB3143
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human HLF (NP_002117.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:1000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human HLF (NP_002117.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEKM SRPL PLNP TFIP PPYG VLRS LLEN PLKL PLHH EDAF SKDK DKEK KLDD ESNS PTVP QSAF LGPT LWDK TLPY DGDT FQLE YMDL EEFL SENG IPPS PSQH DHSP HPPG LQPA SSAA PSVM DLSS RASA PLHP GIPS PNCM QSPI RPGQ LLPA NRNT PSPI DPDT IQVP VGYE PDPA DLAL SSIP GQEM FDPR KRKF SEEE LKPQ PMIK KARK VFIP DDLK DDKY WARR RKNN MAAK RSRD ARRL KENQ IAIR ASFL EKEN SALR QEVA DLRK ELGK CKNI LAKY EARH GPL
Gene ID:	3131
Uniprot:	Q16534
Cellular Location:	Nucleus
Calculated MW:	23kDa/33kDa
Observed MW:	36kDa

Synonyms:

HLF

Background:

This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

UniProt Protein Function:	HLF: A chromosomal aberration involving HLF is a cause of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(17;19)(q22;p13.3) with TCF3. Belongs to the bZIP family. PAR subfamily.
UniProt Protein Details:	Protein type:Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 17q22 Cellular Component: nucleus Molecular Function:DNA binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; multicellular organismal development; rhythmic process
NCBI Summary:	This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16534
NCBI GenInfo Identifier:	6919907
NCBI Gene ID:	3131
NCBI Accession:	Q16534.1
UniProt Secondary Accession:	Q16534,Q6FHS9, A8K1X8,
UniProt Related Accession:	Q16534
Molecular Weight:	295
NCBI Full Name:	Hepatic leukemia factor
NCBI Synonym Full Names:	hepatic leukemia factor
NCBI Official Symbol:	HLF
NCBI Protein Information:	hepatic leukemia factor
UniProt Protein Name:	Hepatic leukemia factor
Protein Family:	Hepatic leukemia factor
UniProt Gene Name:	HLF
UniProt Entry Name:	HLF_HUMAN

	Western blot analysis of extracts of various cell lines, using HLF antibody (CAB3143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using HLF antibody (CAB3143) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)