Anti-HNRNPH2 Antibody (CAB12993)

Antibody Name:	Anti-HNRNPH2 Antibody
Antibody SKU:	CAB12993
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-285 of human HNRNPH2 (NP_062543.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-285 of human HNRNPH2 (NP_062543.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YDRP GAGR GYNS IGRG AGFE RMRR GAYG GGYG GYDD YGGY NDGY GFGS DRFG RDLN YCFS GMSD HRYG DGGS SFQS
Gene ID:	3188
Uniprot:	P55795
Cellular Location:	Nucleus, nucleoplasm
Calculated MW:	49kDa
Observed MW:	49kDa

Synonyms:

HNRNPH2, FTP3, HNRPH2, MRXSB, NRPH2

Background:

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. Read-through transcription between this locus and the ribosomal protein L36a gene has been observed.

UniProt Protein Function:	hnRNP H2: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG).
UniProt Protein Details:	Protein type:RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: Xq22 Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; ribonucleoprotein complex Molecular Function:protein binding; RNA binding Biological Process: gene expression; nuclear mRNA splicing, via spliceosome
NCBI Summary:	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that binds to RNAs. It is very similar to the family member HNRPH1. This gene is thought to be involved in Fabray disease and X-linked agammaglobulinemia phenotype. Alternative splicing results in multiple transcript variants encoding the same protein. Read-through transcription between this locus and the ribosomal protein L36a gene has been observed. [provided by RefSeq, Jan 2011]
UniProt Code:	P55795
NCBI GenInfo Identifier:	2500576
NCBI Gene ID:	3188
NCBI Accession:	P55795.1
UniProt Secondary Accession:	P55795,Q9HHA7, A1L400,
UniProt Related Accession:	P55795
Molecular Weight:	49,264 Da
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein H2
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein H2 (H')
NCBI Official Symbol:	HNRNPH2
NCBI Official Synonym Symbols:	FTP3; HNRPH'; HNRPH2; hnRNPH'
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein H2
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein H2
UniProt Synonym Protein Names:	FTP-3; Heterogeneous nuclear ribonucleoprotein H'; hnRNP H'
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	HNRNPH2
UniProt Entry Name:	HNRH2_HUMAN

	Western blot analysis of extracts of HeLa cells, using HNRNPH2 antibody (CAB12993) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of 293T cells using HNRNPH2 antibody (CAB12993) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ELISA Kits

hnRNP H Colorimetric Cell-Based ELISA

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)