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Western blot analysis of extracts of various cell lines, using at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Key Features

HOOK2 Monoclonal Antibody (CAB9337)

Datasheet MSDS
SKU CAB9337
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms HK2, HOOK2
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

HOOK2 Monoclonal Antibody (CAB9337)

The HOOK2 Monoclonal Antibody (CAB9337) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it an ideal tool for Western blot applications.HOOK2 is a key player in regulating cell division and organelle positioning, making it a crucial target for studies focused on cell biology and cancer research. Understanding the function and regulation of HOOK2 can provide valuable insights into cellular processes and potential therapeutic strategies for diseases like cancer.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HOOK2 Monoclonal Antibody
SKU:CAB9337
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC2769
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MSVD KAEL CGSL LTWL QTFH VPSP CASP QDLS SGLA VAYV LNQI DPSW FNEA WLQG ISED PGPN WKLK VSNL KMVL RSLV EYSQ DVLA HPVS EEHL PDVS
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HK2, HOOK2
Positive Sample:HT-29, 293T, A-431, Mouse brain, Mouse heart, Rat brain
Cellular Localization:Cytoplasm, Centrosome, Cytoskeleton, Microtubule Organizing Center.
Calculated MW:83kDa
Observed MW:90kDa

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.

Purification MethodAffinity purification
Gene ID29911
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HOOK2 Rabbit mAb (CAB9337) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).Exposure time: 3 s.
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