HRP-conjugated Goat anti-Mouse IgG Heavy Chain (CABS064)
The HRP-conjugated Goat anti-Mouse IgG Heavy Chain (CABS064) is a high-quality antibody developed for reliable detection and analysis of target proteins. Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
This antibody is validated for use in WB applications and has demonstrated reactivity against Mouse samples.
Product Name:
HRP-conjugated Goat anti-Mouse IgG Heavy Chain
SKU:
CABS064
Size:
100μL, 20μL, 50μL
Reactivity:
Mouse
Conjugate:
HRP
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:1000 - 1:10000
Positive Sample:
Mouse IgG, Rabbit IgG
Observed MW:
50kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
RRID
AB_2864058
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse and Rabbit IgG, using HRP-conjugated Goat Anti-Mouse IgG Heavy Chain (CABS064) at 1:5000-1:40000 dilution. Lysates/proteins: 100 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
